203189 Biotin-X-NHS, Water-Soluble

203189
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Overview

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203189-50MG
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      Plastic ampoule 50 mg
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      Description
      OverviewReadily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.
      Catalogue Number203189
      Brand Family Calbiochem®
      SynonymsSulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
      References
      ReferencesGretch, D.R., et al. 1987. Anal. Biochem. 163, 270.
      Product Information
      FormOff white to tan solid
      Hill FormulaC₂₀H₂₉N₄O₉S₂ · Na
      Chemical formulaC₂₀H₂₉N₄O₉S₂ · Na
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥94% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Biotin-X-NHS, Water-Soluble SDS

      Title

      Safety Data Sheet (SDS) 

      Biotin-X-NHS, Water-Soluble Certificates of Analysis

      TitleLot Number
      203189

      References

      Reference overview
      Gretch, D.R., et al. 1987. Anal. Biochem. 163, 270.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-May-2008 RFH
      SynonymsSulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
      DescriptionWater solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
      FormOff white to tan solid
      Recommended reaction conditions
      Buffers containing amines (such as Tris or glycine) should be avoided because they can compete with the reaction. It is also very important that the stock solution be prepared just prior to addition of the reaction mixture, because these compounds are prone to hydrolysis by water. Water-soluble crosslinkers can be dissolved in water or buffer at a concentration of 10-25 mM. Phosphate buffers may precipitate sulfo-N-hydroxysuccinimide (sulfo-NHS) containing compounds. In addition, the solid crosslinker can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions, but can be minimized by using more concentrated protein solution (greater than 5 mg/ml). If you prefer to work with stock solutions, we recommend that you dissolve the crosslinker in anhydrous DMSO at a concentration of 10-25 mM. This solution can then be diluted into the buffer of your choice just prior to use. General Handling 1. Store desiccated at 4°C. 2. Warm contents to room temperature completely before opening. Allow at least 30 min. 3. Wipe the vial to remove any moisture. 4. After opening, store under nitrogen. 5. Protect from moisture.
      Chemical formulaC₂₀H₂₉N₄O₉S₂ · Na
      Purity≥94% by HPLC
      SolubilitydH₂O (5.5 mg/ml) or DMSO (13 mg/ml)
      Storage +2°C to +8°C
      Protect from moisture
      Do Not Freeze Ok to freeze
      Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
      Toxicity Standard Handling
      ReferencesGretch, D.R., et al. 1987. Anal. Biochem. 163, 270.