206700 Calcein-AM - CAS 148504-34-1 - Calbiochem

206700
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₄₆H₄₆N₂O₂₃148504-34-1

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206700-1MG
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      Plastic ampoule 1 mg
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      Description
      OverviewA cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein. Can also be used for imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis, and multidrug resistance (MDR).
      Catalogue Number206700
      Brand Family Calbiochem®
      SynonymsCalcein-acetoxymethyl Ester, Diacetate, CAL-AM
      References
      ReferencesDhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
      Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
      Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
      Feller, N., et al. 1995. Br. J. Cancer 72, 543.
      Feller, N., et al. 1995. FEBS Lett. 368, 385.
      Product Information
      CAS number148504-34-1
      ATP CompetitiveN
      FormOff-white solid
      Hill FormulaC₄₆H₄₆N₂O₂₃
      Chemical formulaC₄₆H₄₆N₂O₂₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetImaging mitochondrial permeability pore transition
      Purity≥90% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Calcein-AM - CAS 148504-34-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Calcein-AM - CAS 148504-34-1 - Calbiochem Certificates of Analysis

      TitleLot Number
      206700

      References

      Reference overview
      Dhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
      Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
      Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
      Feller, N., et al. 1995. Br. J. Cancer 72, 543.
      Feller, N., et al. 1995. FEBS Lett. 368, 385.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision31-March-2008 JSW
      SynonymsCalcein-acetoxymethyl Ester, Diacetate, CAL-AM
      DescriptionA cell-permeable, nonfluorescent, and hydrophobic compound, which is rapidly hydrolyzed by cytoplasmic esterases inside the cell, releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein (excitation maximum: 494 nm; emission maximum: 517 nm). A cytosolic fluorophore that can also be used imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, and BCECF and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis and multidrug resistance (MDR).
      FormOff-white solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number148504-34-1
      Chemical formulaC₄₆H₄₆N₂O₂₃
      Structure formulaStructure formula
      Purity≥90% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
      Toxicity Standard Handling
      ReferencesDhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
      Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
      Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
      Feller, N., et al. 1995. Br. J. Cancer 72, 543.
      Feller, N., et al. 1995. FEBS Lett. 368, 385.