208771 Calpain Substrate III, Fluorogenic - Calbiochem

208771
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208771-1MG
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      Description
      OverviewA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 (Cat. Nos. 208712, 208713, 208715, and 208718) based on 106 known cleavage sites. It is cleaved by mammalian calpains with a much better efficacy (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively) and kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in whole Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
      Catalogue Number208771
      Brand Family Calbiochem®
      Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
      References
      ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
      Product Information
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₈₀H₁₁₄N₂₀O₁₈S
      Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetSubstrate sequence that is optimized for calpain-1 and -2
      Primary Target IC<sub>50</sub>kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Calpain Substrate III, Fluorogenic - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Calpain Substrate III, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      208771

      References

      Reference overview
      Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-May-2008 RFH
      Synonyms(DABCYL)-TPLK~SPPPSPR-(EDANS)
      DescriptionA fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2 based on 106 known cleavage sites. It is cleaved by mammalian calpains with higher efficiency (kcat/Kmx10-2 (M-1s-1) = 69.8, 38.5, 20.0, 6.81, 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain, calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively). It is kinetically superior to other commonly used calpain substrates, such as LY-AMC (Cat. No. 208731) and α-spectrin cleavage site-based substrate (Cat. No. 208748). Has been used successfully in monitoring calpain activity in intact Drosophila S2 cells (cellular incorporation achieved with a lipofection reagent) and in COS-7 cell lysate.
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₈₀H₁₁₄N₂₀O₁₈S
      Peptide SequenceDABCYL-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-EDANS
      Purity≥98% by HPLC
      SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Irritant
      ReferencesTompa, P., et al. 2004. J. Biol. Chem. 279, 20775.