204886 Complement C4, Human

204886
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204886-250UG
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      Plastic ampoule 250 μg
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      Description
      OverviewNative, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. of 93 kDa (α), 75 kDa (β), and 32 kDa (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in production of C4a (M.W. 8740) and C4b fragments (M.W. 193 kDa). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
      Catalogue Number204886
      Brand Family Calbiochem®
      References
      ReferencesJanatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
      Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
      Product Information
      CAS number80295-48-3
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activity≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
      Purity≥90% by SDS-PAGE
      SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity>3X10⁵ C4H50 units/mg
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Complement C4, Human SDS

      Title

      Safety Data Sheet (SDS) 

      Complement C4, Human Certificates of Analysis

      TitleLot Number
      204886

      References

      Reference overview
      Janatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
      Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.

      Citations

      Title
    • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-May-2008 RFH
      DescriptionNative, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. 93,000 (α), 75,000 (β), and 32,000 (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in the production of C4a (M.W. 8740) and C4b fragments (M.W. 193,000). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number80295-48-3
      Purity≥90% by SDS-PAGE
      Specific activity>3X10⁵ C4H50 units/mg
      Biological activity≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesJanatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
      Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
      Citation
    • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.