203195 Protein A, Biotin Conjugate

203195
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203195-2MG
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      Plastic ampoule 2 mg
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      Description
      OverviewNative protein A conjugated to biotin.
      Catalogue Number203195
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 1% BSA, pH 7.6.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application NotesSolid Phase Immunoassay (1:10,000)
      Application CommentsThe protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      PurityA single band at MW 50,000 by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protein A, Biotin Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Protein A, Biotin Conjugate Certificates of Analysis

      TitleLot Number
      203195
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-June-2007 RFH
      ApplicationSolid Phase Immunoassay (1:10,000)
      DescriptionNative protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.
      BackgroundLong-chain NHS-Biotin-labeled Protein A is an extremely useful reagent because of its high binding affinity for avidin, streptavidin and avidin- or streptavidin-labeled compounds. Protein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors, and therefore will not give high background reactions.
      FormLiquid
      FormulationIn 10 mM PBS, 1% BSA, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      PurityA single band at MW 50,000 by SDS-PAGE
      Preservative≤0.1% sodium azide
      CommentsThe protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling