Flow Cytometry Kits & Antibodies

FlowCellect Kits & Milli-Mark Fluorescent Conjugated Antibodies

Whether you’re studying cell signaling, cancer, stem cells, immune function, or some other frontier of life science, you’ll quickly be an expert cell analyst with FlowCellect kits and Milli-Mark conjugates. FlowCellect Kits are Merck's proprietary multiparameter kits for cellular analysis. We’ve taken the guesswork out of assay development so you can focus on your results. Each kit provides a unique combination of directly conjugated antibodies and/or fluorescent dyes and protein reporters to monitor changes in protein expression and post-translational modification using flow. The kits also contain complete buffer sets, protocols and pre-defined gate settings. They are fully optimized for "plug-and-play" cellular analysis on Guava EasyCyte and other flow cytometry instruments.

The Milli-Mark fluorescent conjugated antibodies are specifically designed, optimized and validated for flow, exhibit superior signal-to-noise ratios, and eliminate the need for a secondary antibody.

Cell Counting and Viability

Cell counting and viability assessments can be used in a variety of applications, such as cytotoxicity studies, PBMC counting, rapid apoptosis assessment, and increasing reproducibility in cell-based assays. The Guava ViaCount Assay is fast becoming the new standard for viability and cell counting. In this simple no-wash, mix-and-read-assay, you can accurately obtain absolute direct total cell counts without the need for reference beads. Additional benefits include viability assessments, and determine apoptotic percentages— all from tiny samples. You’ll enjoy several advantages over traditional methods, including greater accuracy, reproducibility, and speed.

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Cell Health

Parameters of cell health include apoptosis, oxidative stress, mitochondrial health, DNA integrity, and cell cycle profile.

• Apoptosis, or programmed cell death, is an important regulator of cell growth and proliferation. Breakdowns in the apoptotic pathway can also lead to cancer and various diseases.
  
• Examination of the cell cycle has proven to be an accurate and effective method for assessing cell health. FlowCellect kits make cell cycle studies easier by using a cell cycle phase-specific antibody plus a DNA dye in flow cytometry applications. Bivariate analysis using flow cytometry not only reveals the distribution of cells in particular phases of the cycle, but also helps elucidate the molecular and functional mechanisms associated within the cell cycle.
  
• The loss of mitochondrial membrane potential is an early sign of declining cell health. Multiparametric evaluation of cell populations using FlowCellect kits for mitochondrial health enables detailed kinetic and temporal analysis of the events that lead to apoptosis.

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Immunology

The immune system is made up of multiple cell types collectively called lymphocytes. Lymphocyte subtypes include B cells (which secrete antibodies), cytotoxic T cells, helper T cells (which secrete cytokines), and natural killer (NK) cells. Characterization of lymphocyte subtypes and cytokine signaling is essential for understanding the complex nature of the immune system. Activation by antigens, suppression of normal immune activation, and disease states can affect the phenotypes of lymphocytes. Multiparameter phenotypic analysis with FlowCellect immunology kits allows for a greater understanding of these intricate processes.
As a part of our complete benchtop flow cytometry solution including instruments, software, service, and reagents, our Milli-Mark fluorescently-labeled antibodies for immunology are specifically designed, optimized and validated for flow cytometry applications.

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Cell Signaling

FlowCellect cell signaling kits simultaneously detect key protein activation states and crosstalk using directly conjugated, phospho-specific antibodies. With these kits, you can discern multiple pathway activation and crosstalk in a time-dependent manner, or study the correlation between pathway activation and changes in general cell function and health. In combination with our numerous Milli-Mark fluorescent conjugated antibodies against signaling pathway proteins, our signaling kits and reagents provide a complete solution for multiparameter analysis of pathways such as EGFR/MAPK/Erk, Akt/mTOR/PI3K, Src, and Ras.

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Neuroscience

Merck’s neuro-specific Milli-Mark fluorescent conjugated antibodies grant flow cytometry access to neuroscientists in cancer research and regenerative medicine. Multiparameter flow cytometric cell analysis of neuro-specific markers can help elucidate the differentiation status and cell fate of heterogeneneous cell populations.

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Epigenetics

Over the past decade, the study of epigenetics has shifted from basic mechanisms to their effect on development and disease. Throughout this time, Millipore has been dedicated to developing and refining technologies for the study of epigenetic phenomena. To complement our large portfolio of antibodies and kits for epigenetics and gene regulation, we offer a number of Milli-Mark fluorescent conjugated antibodies to enable the multiparametric correlation of epigenetic regulation with diverse phenotypes in single cells and cell populations.

Click on the Ordering Tab to see our kits and conjugates for epigenetics.