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Conditions

\n\n

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SPME fiber	overcoated PDMS/DVB, 23 gauge
Extraction	immersion, 50 min, 50 °C, 250 rpm, vial penetration 34 mm
Desorption process	3 min, 260°C
Other sample prep information	Sample was incubated prior to extraction for 10 min at 50°C, 400 rpm mixing. \nFiber was washed post-extraction and prior to desorption in water for 0.5 min. \nAfter desorption, fiber was post-baked at 270°C for 6 min.

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Instrument	Agilent^® 7890/7000C GC/MS/MS
Column	SLB-PAHms, 30 m x 0.25 mm I.D., 0.25 µm
Oven	100 °C (3 min), 15 °C/min to 300 °C (10 min)
Inj. temp.	260 °C
Carrier gas	helium, 1 mL/min constant flow
Detector	MS/MS
MSD interface	325 °C
Scan range	MRM: \nBPA: 213/119, 213/91, 119/91 \nBPA-d16: 224/215, 224/97, 125/97
Injection	Splitless
Liner	0.75 mm ID straight (for SPME)

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Description
Analysis Note	Use of bisphenol A (BPA)-based plastics in food and beverage containers continues to be a point of controversy. This study demonstrates the solid phase micro extraction (SPME) of BPA from a fruit flavored energy drink using an overcoated PDMS/DVB fiber (overcoated with PDMS), followed by GC/MS/MS analysis using SLB-PAHms GC Column. At a 10 ppb spiking level, accuracy of the SPME method was 115% with an RSD of 1% for spiked replicates.
Featured Industry	Food & Beverage
Legal Information	Agilent is a trademark of Agilent Technologies, Inc. \nThermogreen is a trademark of Sigma-Aldrich Co. LLC \nRedi-Dri is a trademark of Sigma-Aldrich Co. LLC \nSLB is a trademark of Sigma-Aldrich Co. LLC
Application No.	CLA010218A

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