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CB1046 Anti-CLDN1 Mouse mAb (1C5-D9)

CB1046
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
CB1046-100UG 100 μg
Description
OverviewRecognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.
Catalogue NumberCB1046
Brand Family Calbiochem®
SynonymsAnti-Claudin 1
Application Data
Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from PRC/PRF/5 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (5 µg/ml). Detection: colorimetric (DAB).
References
ReferencesGuevin C, et al. 2009. Antiviral Res. 84, 14.
Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
Stamataki Z, et al. 2009. Blood 113 585.
Farquhar MJ, et al. 2008. J. Virol. 82 8797.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
Clone1C5-D9
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
CB1046-100UG 04055977228410

Documentation

Anti-CLDN1 Mouse mAb (1C5-D9) MSDS

Title

Safety Data Sheet (SDS) 

References

Przegląd literatury
Guevin C, et al. 2009. Antiviral Res. 84, 14.
Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
Stamataki Z, et al. 2009. Blood 113 585.
Farquhar MJ, et al. 2008. J. Virol. 82 8797.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Claudin 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3-5 µg/ml)
Application Data
Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from PRC/PRF/5 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CLDN1 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human CLDN1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-CLDN1 Mouse mAb (1C5-D9) (Cat. No. CB1046) (5 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.
BackgroundCLDN1 is a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations results in neonatal ichthyosis-sclerosing cholangitis syndrome.
HostMouse
ImmunogenFull-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
Clone1C5-D9
IsotypeIgG2a
Specieshuman
Positive controlPRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesGuevin C, et al. 2009. Antiviral Res. 84, 14.
Sainz, B., Jr, et al. 2009. Virol. J. 6, 103.
Stamataki Z, et al. 2009. Blood 113 585.
Farquhar MJ, et al. 2008. J. Virol. 82 8797.