324390 Anti-Dopamine D₁ Receptor Rabbit pAb

324390
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Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Ceny i dostępność

Numer katalogowy DostępnośćOpakowanie Ilość/opak. Cena netto Ilość
324390-100UL
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      Ampulka plastikowa 100 ul
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      Description
      OverviewRecognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
      Catalogue Number324390
      Brand Family Calbiochem®
      References
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.
      Key Applications Immunoblotting (Western Blotting)
      Not Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Free-floating Sections
      Frozen Sections
      Application NotesELISA (see comments)
      Immunoblotting (1:1000)
      Frozen Sections (1:5000)
      Free-Floating Sections (1:5000)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Dopamine D₁ Receptor Rabbit pAb MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dopamine D₁ Receptor Rabbit pAb Certificates of Analysis

      TitleLot Number
      324390

      References

      Przegląd literatury
      Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.

      Citations

      Tytuł
    • Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-July-2007 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1:1000)
      Frozen Sections (1:5000)
      Free-Floating Sections (1:5000)
      Paraffin Sections (not recommended)
      DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlRat brain
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThis antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Toxicity Standard Handling
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Citation
    • Jonathan Kipnis, et al. (2004) Dopamine, through the Extracellular Signal-Related Kinase Pathway, Downregulates CD4+CD25+ Regulatory T-Cell Activity: Implications for Neurodegeneration. Journal of Neuroscience 24, 6133-6143.
    • Application referencesImmunoblotting and Immunohistochemistry Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325. Species Reactivity Kipnis, J., et al. 2004. Journal of Neuroscience 24 6133.