341298 FASTsilver™ Gel Staining Kit

341298
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341298-1KIT
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      Description
      OverviewA rapid and sensitive method for detecting protein and nucleic acids fractionated by PAGE. Provides gels with clear backgrounds and sharp bands. Silver staining is about 100 times more sensitive than the standard Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA.
      Catalogue Number341298
      Brand Family Calbiochem®
      Materials Required but Not Delivered Ethanol
      Glacial acetic acid
      TCA
      Ultra-pure water.
      References
      Product Information
      Kit containsSilver stain, developer, sensitizers I and II, and a user protocol. Note: one kit provides reagents sufficient for staining 25 mini gels.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended useFASTsilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is about 100 times more sensitive than Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ provides gels with exceptionally clear backgrounds and sharp protein or nucleic acid images. The protocol is simple and takes as little as 60 min to yield perfect results.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Storage ConditionsUpon arrival store entire kit contents at room temperature (20°C).
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsSilver stain, developer, sensitizers I and II, and a user protocol. Note: one kit provides reagents sufficient for staining 25 mini gels.
      Specifications

      Documentation

      FASTsilver™ Gel Staining Kit MSDS

      Title

      Safety Data Sheet (SDS) 

      FASTsilver™ Gel Staining Kit Certificates of Analysis

      TitleLot Number
      341298
      User Protocol

      Revision12-April-2011 RFH
      StorageUpon arrival store entire kit contents at room temperature (20°C).
      Intended useFASTsilver™ is one of the most rapid and sensitive methods for detecting proteins and nucleic acids fractionated by PAGE. Staining is about 100 times more sensitive than Coomassie Blue protein staining and about 10 times more sensitive than ethidium bromide for DNA and RNA. FASTsilver™ provides gels with exceptionally clear backgrounds and sharp protein or nucleic acid images. The protocol is simple and takes as little as 60 min to yield perfect results.
      Materials provided(Suitable for 25 mini gels)

      • Silver Stain (Kit component No. KP15701): 1 bottle, 125 ml
      • Developer (Kit component No. KP15702): 1 vial, 75 g
      • Sensitizer-I (Kit component No. KP15703): 1 vial, 4 ml
      • Sensitizer-II (Kit component No. KP15704): 1 vial, 4 ml
      Materials Required but not provided Ethanol
      Glacial acetic acid
      TCA
      Ultra-pure water.
      Reagent preparation• Fixative I: Mix 30 ml 100% ethanol, 10 ml glacial acetic acid, and 60 ml ultra-pure water to obtain 100 ml Fixative I. • Fixative II: Mix 10 ml 100% ethanol and 90 ml ultra-pure water to obtain 100 ml Fixative II. • Sensitizer: Mix 5 ml silver stain, 65 µl sensitizer-I, and 45 ml ultra-pure water to obtain 50 ml Sensitizer. • Developer: Mix 2.5 g developer, 32.5 µl sensitizer-I, 32.5 µl sensitizer-II, and 50 ml ultra-pure water to obtain 50 ml Developer. • Stopper: Mix 11 ml glacial acetic acid and 39 ml ultra-pure water to obtain 50 ml Stopper.
      Detailed protocol1. After electrophoresis, fix the gel in 50 ml of the freshly prepared Fixative I. Use highly purified deionized water to make the solution. For isoelectric focusing gels, fix the gel first in 20% TCA for 60 min.

      2. Fix the gel for 30 min to 3 h depending upon its thickness. For mini gels, (8 x 10 cm), 30 min is sufficient.

      3. Wash twice, 10 min each, in 50 ml of the freshly prepared Fixative II.

      4. Wash three times, 10 min each, in ultra-pure water. During washing Steps 2 and 3, use generous amounts of water (100 ml per 8 x 10 cm mini gels) and use gentle rocking or agitation.

      5. Soak the gel in 50 ml of freshly prepared Sensitizer for 30 min, with gentle rocking of the gel, depending upon the thickness of the gel.

      6. Rinse the gel only for 10-20 s with 50 ml of ultra-pure water.

      7. Soak the gel in 50 ml of freshly prepared Developer. Gently rock the gel until bands are visible. Band intensity will develop quickly.

      8. As soon as band intensity reaches an acceptable level, stop color development by adding 5 ml of the freshly prepared Stopper. Gently rock the gel for 10 min. Store the gel in ultra-pure water for subsequent analysis.
      Sensitivity NotesThe lower detection limit of FASTsilver™ gel stain is 1 ng/band for proteins and nucleic acids.
      Registered TrademarksCalbiochem® is a registered trademark of EMD Chemicals, Inc.
      Interactive Pathways™ is a trademark of EMD Chemicals, Inc.
      FASTsilver™ is a trademark of Geno Technology, Inc.