345731 GDF-5, Mouse, Recombinant

345731
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Przegląd

Replacement Information

Tabela kluczowych gatunków

Purity
≥95% by SDS-PAGE

Ceny i dostępność

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US1345731-50UG
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      Description
      OverviewRecombinant, mouse growth differentiation factor 5 (GDF-5) consisting of amino acids 376-495, expressed in E. coli with and without an N-terminal methionine. GDF-5, a member of the bone morphogenetic protein family, is involved in regulation of myogenesis, bone morphogenesis, neuron differentiation and survival, and chondrogenesis regulation.
      Catalogue Number345731
      Brand Family Calbiochem®
      SynonymsCartilage-derived morphogenetic protein 1 (CDMP-1)
      References
      ReferencesSettle, S.H., Jr., et al. 2003. Dev. Biol. 254, 116.
      Massague, J., et al. 2000. Genes and Dev. 14, 627.
      FrancisWest, P.H., et al. 1999.
      Development 126, 1035. Inada, M., et al. 1996. BBRC 222, 317.
      Nishitoh, H., et al. 1996. J. Biol. Chem. 271, 21345.
      Storm, E.E., et al. 1994. Nature 368, 639.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm filter-sterilized Acetonitrile, TFA, BSA.
      Quality LevelMQ100
      Applications
      ApplicationGDF-5, Mouse, Recombinant, consists of amino acids 376-495 and is expressed in E. coli with and without an N-terminal methionine. Involved in myogenesis, bone morphogenesis & neuron differentiation.
      Biological Information
      Biological activityED₅₀ = 1-4 µg/ml as measured by induction of alkaline phosphatase production by ATDC5 mouse chondrogenic cells. (Nakamura, K. et al. 1999. Exp. Cell Res. 250, 351.)
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      GDF-5, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      345731

      References

      Przegląd literatury
      Settle, S.H., Jr., et al. 2003. Dev. Biol. 254, 116.
      Massague, J., et al. 2000. Genes and Dev. 14, 627.
      FrancisWest, P.H., et al. 1999.
      Development 126, 1035. Inada, M., et al. 1996. BBRC 222, 317.
      Nishitoh, H., et al. 1996. J. Biol. Chem. 271, 21345.
      Storm, E.E., et al. 1994. Nature 368, 639.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2011 RFH
      SynonymsCartilage-derived morphogenetic protein 1 (CDMP-1)
      DescriptionRecombinant, mouse growth differentiation factor 5 (GDF-5) consisting of amino acids 376-495, expressed in E. coli with and without an N-terminal methionine. GDF-5, a member of the bone morphogenetic protein family, is involved in regulation of myogenesis, bone morphogenesis, neuron differentiation and survival, and chondrogenesis regulation.
      FormSolid
      FormulationLyophilized from 0.2 µm filter-sterilized Acetonitrile, TFA, BSA.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Biological activityED₅₀ = 1-4 µg/ml as measured by induction of alkaline phosphatase production by ATDC5 mouse chondrogenic cells. (Nakamura, K. et al. 1999. Exp. Cell Res. 250, 351.)
      SolubilityReconstitute to 150 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesSettle, S.H., Jr., et al. 2003. Dev. Biol. 254, 116.
      Massague, J., et al. 2000. Genes and Dev. 14, 627.
      FrancisWest, P.H., et al. 1999.
      Development 126, 1035. Inada, M., et al. 1996. BBRC 222, 317.
      Nishitoh, H., et al. 1996. J. Biol. Chem. 271, 21345.
      Storm, E.E., et al. 1994. Nature 368, 639.