345762 GDF-8/Myostatin, Human, Mouse, Rat, Recombinant

345762
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Przegląd

Replacement Information

Tabela kluczowych gatunków

Purity
≥80% by SDS-PAGE

Ceny i dostępność

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US1345762-10UG
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      Butelka szklana 10 μg
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      Description
      OverviewRecombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
      Catalogue Number345762
      Brand Family Calbiochem®
      SynonymsMyostatin
      References
      ReferencesKim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
      Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
      Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
      McPherron, A.C., et al. 1997. Nature 387, 83.
      Storm, E.E., et al. 1994. Nature 368, 639.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
      Quality LevelMQ100
      Applications
      ApplicationGDF-8/Myostatin, Human, Mouse, Rat, Recombinant, consists of amino acids 268-376 and is expressed in NS0-derived murine myeloma cells. Useful for inhibiting preadipocyte differentiation to adipocytes.
      Biological Information
      Biological activityHemoglobin Expression:
      ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.

      Elongation of Animal Caps:
      Optimal Elongation of animal caps from Xenpus blastulae.
      Purity≥80% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU per/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      GDF-8/Myostatin, Human, Mouse, Rat, Recombinant Certificates of Analysis

      TitleLot Number
      345762

      References

      Przegląd literatury
      Kim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
      Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
      Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
      McPherron, A.C., et al. 1997. Nature 387, 83.
      Storm, E.E., et al. 1994. Nature 368, 639.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2011 RFH
      SynonymsMyostatin
      DescriptionRecombinant, human mouse rat growth differentiation factor 8 (GDF-8) consisting of amino acids 268-376 and expressed in NS0-derived murine myeloma cells. GDF-8 is expressed in developing and adult skeletal muscle and is 100% identical to mature human, rat, mouse, and cow at the amino acid sequence level. Useful for the inhibition of preadipocyte differentiation to adipocytes.
      FormSolid
      FormulationLyophilized from 0.2 µm filtered Acetonitrile, TFA, BSA.
      Purity≥80% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU per/µg protein
      Biological activityHemoglobin Expression:
      ED₅₀ = 2-10 ng/ml as measured by induction of hemoglobin expression in k562 human chronic myelogenous leukemia cells.

      Elongation of Animal Caps:
      Optimal Elongation of animal caps from Xenpus blastulae.
      SolubilityReconstitute to 100 µg/ml in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesKim, H.S., et al. 2001. Biochem. Biophys. Res. Commun. 281, 902.
      Lee, S.J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 9306.
      Sharma, M., et al. 1999. J. Cell Physiol. 180, 1.
      McPherron, A.C., et al. 1997. Nature 387, 83.
      Storm, E.E., et al. 1994. Nature 368, 639.