376811 Hexokinase, Yeast

376811
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376811-2500IU
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      Description
      OverviewNative hexokinase from yeast. Catalyzes the phosphorylation of D-glucose to glucose-6-phosphate.
      Catalogue Number376811
      Brand Family Calbiochem®
      SynonymsHK
      References
      ReferencesDarrow, R.A., and Colowick, S.P. 1962. Methods Enzymol. 5, 226.
      McDonald, M.R. 1955. Methods Enzymol. 1, 269.
      Product Information
      CAS number9001-51-8
      Activity≥80 units/mg dry weight.
      Unit of DefinitionOne unit is defined as the amount of enzyme that will phosphorylate 1.0 µmol D-hexose in the presence of ATP per min at 30°C, pH 7.5.
      EC number2.7.1.1
      FormLyophilized solid
      FormulationSalt-free.
      PI4.5 - 4.8
      Quality LevelMQ100
      Applications
      ApplicationHexokinase, Yeast, CAS 9001-51-8, catalyzes the phosphorylation of D-glucose to glucose-6-phosphate.
      Biological Information
      Specific Activity≥150 units/mg
      Physicochemical Information
      Contaminants6-PGDH: ≤0.01%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Hexokinase, Yeast Certificates of Analysis

      TitleLot Number
      376811

      References

      Przegląd literatury
      Darrow, R.A., and Colowick, S.P. 1962. Methods Enzymol. 5, 226.
      McDonald, M.R. 1955. Methods Enzymol. 1, 269.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-August-2011 JSW
      SynonymsHK
      DescriptionNative hexokinase from yeast. Catalyzes the phosphorylation of D-glucose to glucose-6-phosphate. Has an optimal pH of 7.6-9.0 and a pI of 4.5-4.8.
      FormLyophilized solid
      FormulationSalt-free.
      CAS number9001-51-8
      EC number2.7.1.1
      Contaminants6-PGDH: ≤0.01%
      Specific activity≥150 units/mg
      Activity≥80 units/mg dry weight.
      Unit definitionOne unit is defined as the amount of enzyme that will phosphorylate 1.0 µmol D-hexose in the presence of ATP per min at 30°C, pH 7.5.
      SolubilityTris-HCl buffer, pH 8.0, containing 5-10 mM MgCl₂
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
      Toxicity Standard Handling
      ReferencesDarrow, R.A., and Colowick, S.P. 1962. Methods Enzymol. 5, 226.
      McDonald, M.R. 1955. Methods Enzymol. 1, 269.