17-283 Rac1 Activation Assay Kit

17-283
1 kit  Kit capacity: 30 assays
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      Replacement Information

      Tabela kluczowych gatunków

      Species ReactivityKey Applications
      H, M, R ABA
      Description
      Catalogue Number17-283
      ReplacesSGT425
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRac1 Activation Assay Kit
      References
      Product Information
      Components
      • Anti-Rac1, clone 23A8 (Cat.# 05-389)
      • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
      Quality LevelMQ100
      Applications
      ApplicationNon-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
      Gene Symbol
      • RAC1
      • MGC111543
      • ORFNames=MIG5
      • PAK1
      • MIG5
      • TC-25
      • p21-Rac1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
      SIZE: 192 amino acids; 21450 Da
      SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
      DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
      SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 30 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Rac1 Activation Assay Kit MSDS

      Title

      Safety Data Sheet (SDS) 

      Rac1 Activation Assay Kit Certificates of Analysis

      TitleLot Number
      Rac Activation Kit - 1894318943
      Rac1 Activation Assay Kit3043198
      Rac1 Activation Assay Kit2476980
      Rac1 Activation Assay Kit (non-radioactive) - 21530972153097
      Rac1 Activation Assay Kit (non-radioactive) - 23821402382140
      Rac1 Activation Assay Kit (non-radioactive)3125679
      Rac1 Activation Assay Kit (non-radioactive)2986309
      Rac1 Activation Assay Kit (non-radioactive) - 22505092250509
      Rac1 Activation Assay Kit (non-radioactive) - 23290512329051
      Rac1 Activation Assay Kit (non-radioactive) -24996852499685

      References

      Reference overviewApplicationSpeciesPub Med ID
      The role of mechanical stretching in the activation and localization of adhesion proteins and related intracellular molecules.
      Boccafoschi, F, et al.
      J. Cell. Biochem., 112: 1403-9 (2011)  2011

      Pokaż streszczenie
      21321993 21321993
      Focal adhesion assembly in myofibroblasts fosters a microenvironment that promotes tumor growth.
      Kang N, Yaqoob U, Geng Z, Bloch K, Liu C, Gomez T, Billadeau D, Shah V
      Am J Pathol  177  1888-900. Epub 2010 Aug 27.  2010

      Pokaż streszczenie Pełny tekst artykułu
      20802179 20802179
      Edaravone mimics sphingosine-1-phosphate-induced endothelial barrier enhancement in human microvascular endothelial cells.
      Omori, Kazuyoshi, et al.
      Am. J. Physiol., Cell Physiol., 293: C1523-31 (2007)  2007

      17686998 17686998
      Nox1-dependent reactive oxygen generation is regulated by Rac1
      Cheng, Guangjie, et al.
      J. Biol. Chem., 281:17718-17726 (2006)  2006

      Human16636067 16636067
      Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
      Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar, Marek Honczarenko, Michael Mills, Jens Wanzeck, Anna Janowska-Wieczorek, Mariusz Z Ratajczak
      Blood  105  40-8  2005

      Pokaż streszczenie
      15328152 15328152
      Fc{epsilon}RI-mediated mast cell degranulation requires calcium-independent microtubule-dependent translocation of granules to the plasma membrane.
      Nishida, Keigo, et al.
      J. Cell Biol., 170: 115-26 (2005)  2005

      Pokaż streszczenie
      Activation Assay15998803 15998803
      The survival kinase Mirk/dyrk1B is activated through Rac1-MKK3 signaling.
      Kideok Jin, Seunghwan Lim, Stephen E Mercer, Eileen Friedman
      The Journal of biological chemistry  280  42097-105  2005

      Pokaż streszczenie
      16257974 16257974
      FARP2 triggers signals for Sema3A-mediated axonal repulsion.
      Toyofuku, Toshihiko, et al.
      Nat. Neurosci., 8: 1712-9 (2005)  2005

      16286926 16286926
      CD28-dependent HIV-1 Transcription Is Associated with Vav, Rac, and NF-{kappa}B Activation
      Cook, J. A., et al
      J Biol Chem, 278:35812-35818 (2003)  2003

      Activation Assay12842899 12842899
      Pitx2a expression alters actin-myosin cytoskeleton and migration of HeLa cells through Rho GTPase signaling.
      Wei, Qize and Adelstein, Robert S
      Mol. Biol. Cell, 13: 683-97 (2002)  2002

      Pokaż streszczenie
      Activation Assay11854422 11854422

      Brochure

      Title
      Hallmarks of Aging

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      Kit Component

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      14-325 Rac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 µg Pokaż ceny i dostępność
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      Kategorie

      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays