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171591 β-Amyloid Peptide (1-40), Human, Fluorescent-Labeled

171591
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





An N-terminally modified human Aβ1-40 peptide labeled with a fluorophore, 7-diethylaminocoumarin-3-carbonyl (DAC) that shares a common binding site with Aβ1-40 (Cat. No. 171590) with 40-fold greater affinity. Displays stronger affinity towards GM1-ganglioside and cholesterol containing membranes but little affinity for phospholipids. Exhibits only a weak fluorescence in aqueous medium; however, it strongly fluoresces upon binding to membranes. Useful in assessing the binding affinity of Aβ for various membranes.
Catalogue Number171591
Brand Family Calbiochem®
SynonymsDAC-Aβ1-40, Human
References
ReferencesKakio, A., et al. 2002. Biochemistry 41, 7385.
Kakio, A., et al. 2001. J. Biol. Chem. 276, 24985.
Mizuno, T., et al. 1999. J. Biol. Chem. 274, 15110.
Product Information
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₂₀₈H₃₀₈N₅₄O₆₁S
Chemical formulaC₂₀₈H₃₀₈N₅₄O₆₁S
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -70°C. Stock solutions are stable for up to 1 month at -70°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

β-Amyloid Peptide (1-40), Human, Fluorescent-Labeled Certificates of Analysis

TitleLot Number
171591

References

Reference overview
Kakio, A., et al. 2002. Biochemistry 41, 7385.
Kakio, A., et al. 2001. J. Biol. Chem. 276, 24985.
Mizuno, T., et al. 1999. J. Biol. Chem. 274, 15110.

Brochure

Title
Alzheimer's Disease Brochure & Technical Guide
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-April-2008 RFH
SynonymsDAC-Aβ1-40, Human
DescriptionAn N-terminally modified human Aβ1-40 peptide labeled with a fluorophore, 7-diethylaminocoumarin-3-carbonyl (DAC), which shares a common binding site with Aβ1-40 (Cat. No. 171590). However, it displays about 40-fold greater affinity than Aβ1-40. Exhibits only a weak fluorescence in aqueous medium; however, it fluoresces strongly upon binding to the membranes. Displays greater affinity towards the GM1-ganglioside and cholesterol-containing membranes but little affinity for phospholipids. A reduction in GM1 content is reported to reduce DAC-Aβ1-40 binding to membranes. DAC fluorescence can be used to assess the binding affinity of Aβ for various membranes. Excitation max.: ~430 nm; emission max.: ~470 nm.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₀₈H₃₀₈N₅₄O₆₁S
Structure formulaStructure formula
Purity≥95% by HPLC
Solubility25% Acetic Acid (1 mg/ml) or DMSO (5 mg/ml)
Storage ≤ -70°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -70°C. Stock solutions are stable for up to 1 month at -70°C.
Toxicity Standard Handling
ReferencesKakio, A., et al. 2002. Biochemistry 41, 7385.
Kakio, A., et al. 2001. J. Biol. Chem. 276, 24985.
Mizuno, T., et al. 1999. J. Biol. Chem. 274, 15110.