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194932 AZOCOLL™ Substrate, <50 Mesh

194932
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
194932-1GM Plastic ampoule 1 gm
Description
Overview

This product has been discontinued.





General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.2. Mesh: <50 (minimum 80%).
Catalogue Number194932
Brand Family Calbiochem®
References
ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Moore, G.L. 1969. Anal. Biochem. 32, 122.
Product Information
CAS number53092-90-3
FormDark red solid
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

AZOCOLL™ Substrate, <50 Mesh SDS

Title

Safety Data Sheet (SDS) 

AZOCOLL™ Substrate, <50 Mesh Certificates of Analysis

TitleLot Number
194932

References

Reference overview
Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Moore, G.L. 1969. Anal. Biochem. 32, 122.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-November-2009 RFH
DescriptionGeneral proteolytic substrate. Provides a rapid and simple spectrophotometric method to detect the presence of proteases in aqueous solutions. As the insoluble substrate is cleaved by proteases, it releases a red dye into the surrounding buffer. Incubation of 50 mg AZOCOLL® Substrate with 2 units of PRONASE® Protease for 15 min yields an absorbance of greater than 1.0 at 520 nm.
FormDark red solid
CAS number53092-90-3
SolubilityH₂O or Aqueous Buffers
Storage +15°C to +30°C
Protect from light
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesPoilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Moore, G.L. 1969. Anal. Biochem. 32, 122.