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102555 Abl Protein Tyrosine Kinase, Recombinant, E. coli

102555
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, truncated form of the v-Abl protein tyrosine kinase that contains the minimum region required for both kinase activity and fibroblast transformation expressed in E. coli. The catalytic domain of v-Abl is identical to that of c-Abl. Induces resistance to anti-cancer drugs by suppressing apoptosis, probably by upregulating Bcl-xL protein.

Catalogue Number102555
Brand Family Calbiochem®
Synonymsv-Abl
References
ReferencesChen, Q., et al. 1997. Oncogene 15, 2249.
Chapman, R.S., et al. 1995. Mol. Pharmacol. 48, 334.
Evans, C.A., et al. 1995. J. Cell Sci. 108, 2591.
Foulkes, J.G., et al. 1985. J. Biol. Chem. 260, 8070.
Product Information
Activity≥100,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to EAIYAAPFAKKK per min at 30°C, pH 7.5.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Abl Protein Tyrosine Kinase, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Abl Protein Tyrosine Kinase, Recombinant, E. coli Certificates of Analysis

TitleLot Number
102555

References

Reference overview
Chen, Q., et al. 1997. Oncogene 15, 2249.
Chapman, R.S., et al. 1995. Mol. Pharmacol. 48, 334.
Evans, C.A., et al. 1995. J. Cell Sci. 108, 2591.
Foulkes, J.G., et al. 1985. J. Biol. Chem. 260, 8070.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-June-2009 JSW
Synonymsv-Abl
DescriptionRecombinant, truncated form of the v-Abl protein tyrosine kinase that contains the minimum region required for both kinase activity and fibroblast transformation expressed in E. coli. The catalytic domain of v-abl is identical to that of c-abl. Induces resistance to anti-cancer drugs by suppressing apoptosis. Also involved in the upregulation of Bcl-xL protein.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions1x kinase buffer: 50 mM Tris-HCl, 10 mM MgCl2, 100 µM EDTA, 1 mM DTT, 100 µM ATP (γ-ATP to 100 µCi/µmol), 0.015% BRIJ® 35 detergent, and 100 µg/ml BSA. Incubate at 30°C. Note: optimal reaction conditions need to be established empirically for each substrate.
Activity≥100,000 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to EAIYAAPFAKKK per min at 30°C, pH 7.5.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesChen, Q., et al. 1997. Oncogene 15, 2249.
Chapman, R.S., et al. 1995. Mol. Pharmacol. 48, 334.
Evans, C.A., et al. 1995. J. Cell Sci. 108, 2591.
Foulkes, J.G., et al. 1985. J. Biol. Chem. 260, 8070.