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164598 Aminopeptidase M, Porcine Kidney

164598
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
164598-50U Plastic ampoule 50 u
Description
OverviewNative amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
Catalogue Number164598
Brand Family Calbiochem®
SynonymsAminopeptidase M
References
ReferencesWachsmuth, E.E., et al. 1966. Biochemistry 5, 169.
Product Information
CAS number9054-63-1
Activity≥50 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-<i>p</i>NA per min at 37°C, pH 7.2.
EC number3.4.11.2
FormWhite crystalline suspension
FormulationCrystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥10 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsOxidases: ≤3%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Aminopeptidase M, Porcine Kidney SDS

Title

Safety Data Sheet (SDS) 

Aminopeptidase M, Porcine Kidney Certificates of Analysis

TitleLot Number
164598

References

Reference overview
Wachsmuth, E.E., et al. 1966. Biochemistry 5, 169.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-April-2011 JSW
SynonymsAminopeptidase M
DescriptionNative amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
FormWhite crystalline suspension
FormulationCrystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number9054-63-1
EC number3.4.11.2
ContaminantsOxidases: ≤3%
Specific activity≥10 units/mg protein
Activity≥50 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.
SolubilityH₂O or physiological buffers
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Toxicity Harmful
ReferencesWachsmuth, E.E., et al. 1966. Biochemistry 5, 169.

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Categories

Life Science Research > Proteins and Enzymes > Other Enzymes