Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

171537 Anti-Amyloid Precursor Protein Mouse mAb (3E9)

171537
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~110-135 kDa amyloid precursor protein (APP695).
  • Antibody Target Gene Symbol: APP
  • Target Synonym: AAA, AD1, Adap, AL024401, Amyloid precursor, Amyloidogenic glycoprotein, APP isoform 1, APPI, appican, CTFgamma, CVAP, E030013M08RIK, Nexin II, P3, PN2, PreA4, PROTEASE NEXIN2
  • Entrez Gene Name: amyloid beta (A4) precursor protein
  • Hu Entrez ID: 351 (Related Antibodies: 171610, PC729,PC152, PC151, PC150, PC149,NE1012, 171609, 171608, 171607, 171606, 171605, 171604, 171603, 171598)
  • Mu Entrez ID: 11820
  • Rat Entrez ID: 54226
  • Catalogue Number171537
    Brand Family Calbiochem®
    References
    Product Information
    FormLyophilized
    FormulationLyophilized from PBS, 1% sucrose.
    Preservative≤0.1% sodium azide
    Applications
    Key Applications Immunoblotting (Western Blotting)
    Paraffin Sections
    Application NotesImmunoblotting (5-10 µg/ml)
    Paraffin Sections (5-10 µg/ml, see comments)
    Application CommentsThis antibody may not detect APP in normal brain tissue, or APP in samples from later-stage Alzheimer's brain tissue. Suitable for formalin fixed paraffin sections. Variables associated with assay conditions will dictate the proper working dilutions.
    Biological Information
    Immunogena synthetic peptide (LEVPTDGNAGLLAEPQIAMFC) corresponding to amino acids 18-38 of human amyloid precursor protein (APP₆₉₅)
    ImmunogenHuman
    Clone3E9
    HostMouse
    IsotypeIgG₁
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Ambient Temperature Only
    Toxicity Standard Handling
    Storage +2°C to +8°C
    Do not freeze Ok to freeze
    Special InstructionsReconstitute with dH₂O. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications

    Documentation

    Anti-Amyloid Precursor Protein Mouse mAb (3E9) Certificates of Analysis

    TitleLot Number
    171537

    Brochure

    Title
    Alzheimer's Disease Brochure & Technical Guide
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-July-2007 RFH
    ApplicationImmunoblotting (5-10 µg/ml)
    Paraffin Sections (5-10 µg/ml, see comments)
    DescriptionMouse monoclonal antibody purified by ammonium sulfate precipitation and protein A chromatography. Recognizes the ~110-135 kDa amyloid precursor (APP) protein.
    BackgroundAmyloid β protein (or A4) is the major protein subunit of amyloid fibrils and deposits characteristic of the neurodegenerative disorder Alzheimer's disease. The 4.5 kDa A4 protein is derived from proteolytic cleavage of amyloid precursor protein (APP). Three types of APPs (APP695, APP751, APP770), derived from mRNAs generated by alternatively spliced transcripts, are reported. The primary structure of APP695 (the 695 residue polypeptide) indicates that it is a glycosylated protein that spans the membrane once and has a large extracellular domain and a small cytoplasmic domain. The A4 protein, comprised of residues 597-638 of APP695, includes 28 residues from the extracellular domain and 14-15 residues from the transmembrane domain. This monoclonal anti-APP antibody is specific for human APP.
    HostMouse
    Immunogen speciesHuman
    Immunogena synthetic peptide (LEVPTDGNAGLLAEPQIAMFC) corresponding to amino acids 18-38 of human amyloid precursor protein (APP₆₉₅)
    Clone3E9
    IsotypeIgG₁
    Specieshuman
    FormLyophilized
    FormulationLyophilized from PBS, 1% sucrose.
    Preservative≤0.1% sodium azide
    CommentsThis antibody may not detect APP in normal brain tissue, or APP in samples from later-stage Alzheimer's brain tissue. Suitable for formalin fixed paraffin sections. Variables associated with assay conditions will dictate the proper working dilutions.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsReconstitute with dH₂O. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
    Toxicity Standard Handling