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202000 Anti-Bim (22-40) Rabbit pAb

202000
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.



We are offering Anti-Bim Antibody, internal epitope, pan-Bim isoforms (Cat. No. AB17003) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






Recognizes the ~23 kDa Bim protein in K562 and A549 cells.
Catalogue Number202000
Brand Family Calbiochem®
SynonymsAnti-BOD
Application Data
Detection of human Bim by immunoblotting. Samples: Whole cell lysate from K562 cells at primary antibody dilutions 2.5 µg/ml (lane A) and µg/ml (lane B). Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (5 µg/ml). Detection: chemiluminescence.

Detection of human Bim by immunocytochemistry. Sample: K562 cells. Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (10 µg/ml). Detection: fluorescence.
References
ReferencesHsu, S.Y., et al. 1998. Mol. Endocrinol. 12, 1432.
O’Connor, L., et al. 1998. EMBO J. 17, 384.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlK562 or A549 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunocytochemistry Kurinna, S.M., et al. 2004. Cancer Res. 64, 7852.
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1:1000)
Immunocytochemistry (see application references)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(C)AERPPQLRPGAPTSLQTEP] corresponding to amino acids 22-40 of human Bim, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long term storage. Aliquots may be stored at 4°C for up to 1 year.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Bim (22-40) Rabbit pAb Certificates of Analysis

TitleLot Number
202000

References

Reference overview
Hsu, S.Y., et al. 1998. Mol. Endocrinol. 12, 1432.
O’Connor, L., et al. 1998. EMBO J. 17, 384.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Svitlana M. Kurinna, et al. (2004) Ceramide Promotes Apoptosis in Lung Cancer-Derived A549 Cells by a Mechanism Involving c-Jun NH2-Terminal Kinase. Cancer Research 64, 7852-7856.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-June-2007 RFH
    SynonymsAnti-BOD
    ApplicationImmunoblotting (1:1000)
    Immunocytochemistry (see application references)
    Application Data
    Detection of human Bim by immunoblotting. Samples: Whole cell lysate from K562 cells at primary antibody dilutions 2.5 µg/ml (lane A) and µg/ml (lane B). Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (5 µg/ml). Detection: chemiluminescence.

    Detection of human Bim by immunocytochemistry. Sample: K562 cells. Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (10 µg/ml). Detection: fluorescence.
    DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~23 kDa Bim protein.
    BackgroundMembers of the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. The Bcl-2 homology 3 (BH3) domain is a potent death domain and BH3 domain-containing pro-apoptotic proteins, such as Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain-containing protein that induces apoptosis was identified and designated Bim or BOD. Bim/BOD interacts with diverse members in the pro-survival Bcl-2-subfamily, including Bcl-2, Bcl-xL, and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide [(C)AERPPQLRPGAPTSLQTEP] corresponding to amino acids 22-40 of human Bim, conjugated to KLH
    IsotypeIgG
    Specieshuman, mouse, rat
    Positive controlK562 or A549 cells
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsVariables associated with assay conditions will dictate the optimal working dilution.
    Storage -20°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long term storage. Aliquots may be stored at 4°C for up to 1 year.
    Toxicity Standard Handling
    ReferencesHsu, S.Y., et al. 1998. Mol. Endocrinol. 12, 1432.
    O’Connor, L., et al. 1998. EMBO J. 17, 384.
    Citation
  • Svitlana M. Kurinna, et al. (2004) Ceramide Promotes Apoptosis in Lung Cancer-Derived A549 Cells by a Mechanism Involving c-Jun NH2-Terminal Kinase. Cancer Research 64, 7852-7856.
  • Application referencesImmunocytochemistry Kurinna, S.M., et al. 2004. Cancer Res. 64, 7852.