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217575 Anti-CD4 Mouse mAb (QS4120)

217575
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes domain 1 of CD4.
Catalogue Number217575
Brand Family Calbiochem®
References
ReferencesHealey, D., et al. 1990. J. Exp. Med. 172, 1233.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative0.5 mg/ml gentamycin sulfate
Applications
Data setup Error. Pagelet <collection_feature_application_id-2D PAGE> not found.
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Frozen Sections
Application Notes2D Gel Electrophoresis (see comments)
ELISA (see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Application CommentsFACS was performed on 5x105 HPB-MLT human tumor cells and analyzed by flow cytometry. Optimal binding was obtained at 5 µg/ml using goat anti-mouse IgG, FITC conjugated, as the secondary antibody. This antibody has also been reported to work for 2D gel electrophoresis, ELISA, and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenCD4+ transfectant/human CEM
ImmunogenHuman
CloneQS4120
HostMouse
Purity≥95%
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD4 Mouse mAb (QS4120) Certificates of Analysis

TitleLot Number
217575

References

Reference overview
Healey, D., et al. 1990. J. Exp. Med. 172, 1233.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
Application2D Gel Electrophoresis (see comments)
ELISA (see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes domain 1 of CD4.
BackgroundThe amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120 and this antibody will block binding of HIV-1 gp120 to CD4. It also blocks HLA Class II Rosette formation.
HostMouse
Immunogen speciesHuman
ImmunogenCD4+ transfectant/human CEM
CloneQS4120
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95%
Preservative0.5 mg/ml gentamycin sulfate
CommentsFACS was performed on 5x105 HPB-MLT human tumor cells and analyzed by flow cytometry. Optimal binding was obtained at 5 µg/ml using goat anti-mouse IgG, FITC conjugated, as the secondary antibody. This antibody has also been reported to work for 2D gel electrophoresis, ELISA, and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesHealey, D., et al. 1990. J. Exp. Med. 172, 1233.