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217604 Anti-CD44v6 Rabbit pAb

217604
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~200-250 kDa CD44v6 protein in MCF-7 cells.
Catalogue Number217604
Brand Family Calbiochem®
SynonymsAnti-CD44 Variant Isoform with Exon-10 (v6)
References
ReferencesDall, P., et al. 1994. Cancer Res. 54, 3337
Arch, R., et al. 1992. Science 257, 682
Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 12160.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Positive controlMCF-7 or other breast cancer cell line
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Radioimmunoassay
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Frozen Sections
Application NotesELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:10,000)
Frozen Sections (1:250)
Paraffin Sections (1:250)
Immunoprecipitation (1:200)
Radioimmunoassay (1:5000)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from human CD44v6
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD44v6 Rabbit pAb Certificates of Analysis

TitleLot Number
217604

References

Reference overview
Dall, P., et al. 1994. Cancer Res. 54, 3337
Arch, R., et al. 1992. Science 257, 682
Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 12160.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-CD44 Variant Isoform with Exon-10 (v6)
ApplicationELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:10,000)
Frozen Sections (1:250)
Paraffin Sections (1:250)
Immunoprecipitation (1:200)
Radioimmunoassay (1:5000)
DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and DEAE column chromatography. Recognizes the ~200-250 kDa CD44v6 protein.
BackgroundCD44 is a cell surface glycoprotein expressed in a wide variety of tissues and in multiple isoforms resulting from both alternative transcript splicing and differential glycosylation. Some isoforms are believed to be involved in tumor growth and metastasis. CD44v6 is an isoform that has been implicated in tumor metastasis.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from human CD44v6
IsotypeIgG
Speciesbovine, canine, human, mouse, porcine, rabbit
Positive controlMCF-7 or other breast cancer cell line
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDall, P., et al. 1994. Cancer Res. 54, 3337
Arch, R., et al. 1992. Science 257, 682
Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 12160.