Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

217677 Anti-CD54 Mouse mAb (8.4A6)

217677
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
Catalogue Number217677
Brand Family Calbiochem®
SynonymsAnti-ICAM-1, Domain 2
References
ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell 68, 71.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Negative controlHPB-MLT cells
Positive controlRaji cells
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunoprecipitation
Cell Inhibition Assays
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Application NotesELISA (see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
Application CommentsThe antibody recognizes the D2 domain of ICAM-1 and inhibits ICAM-1 binding to LFA-1. Also reported to work for ELISA, frozen sections, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman endothelial cells
ImmunogenHuman
Clone8.4A6
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD54 Mouse mAb (8.4A6) Certificates of Analysis

TitleLot Number
217677

References

Reference overview
Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell 68, 71.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-ICAM-1, Domain 2
ApplicationELISA (see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Cell Inhibition Assays (see comments)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
BackgroundHuman CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak, but is upregulated on activated T and B lymphocytes and monocytes.
HostMouse
Immunogen speciesHuman
Immunogenhuman endothelial cells
Clone8.4A6
IsotypeIgG₁
Specieshuman
Positive controlRaji cells
Negative controlHPB-MLT cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe antibody recognizes the D2 domain of ICAM-1 and inhibits ICAM-1 binding to LFA-1. Also reported to work for ELISA, frozen sections, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
Berendt, A.R., et al. 1992. Cell 68, 71.