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208903 Anti-Calpastatin, Domain IV Mouse mAb (1F7E3D10)

208903
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~150, ~125, ~90, and ~70 kDa forms of calpastatin that are believed to correspond to proteins translated from different start sites of the calpastatin gene.
Catalogue Number208903
Brand Family Calbiochem®
References
ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.
Lane, R.D., et al. 1992. Exper. Cell Res. 203, 5.
Product Information
FormLiquid
FormulationAscites diluted in PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting (1:5000, colorimetric)
Immunofluorescence (1:200)
Application CommentsRecognizes multiple forms of calpastatin that are suggested to be a result of translation initiation from different start sites. Epitope is in domain IV (543-673) of human calpastatin. Reacts with calpastatin from several mammalian sources. Does not cross-react with calpain or calmodulin. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified calpastatin from bovine skeletal muscle
ImmunogenBovine
Clone1F7E3D10
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Calpastatin, Domain IV Mouse mAb (1F7E3D10) Certificates of Analysis

TitleLot Number
208903

References

Reference overview
Cong, J., et al. 1993. J. Biol. Chem. 268, 25740.
Lane, R.D., et al. 1992. Exper. Cell Res. 203, 5.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-July-2007 RFH
ApplicationImmunoblotting (1:5000, colorimetric)
Immunofluorescence (1:200)
DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x3Ag8.653 mouse myeloma cells. Supplied as diluted ascites. Recognizes the ~150, ~125, ~90, and ~70 kDa forms of calpastatin.
BackgroundCalpastatin is a specific endogenous inhibitor of calpain that has an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds Ca2+. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.
HostMouse
Immunogen speciesBovine
Immunogenpurified calpastatin from bovine skeletal muscle
Clone1F7E3D10
IsotypeIgG₁
Speciesbovine, human, rat
FormLiquid
FormulationAscites diluted in PBS.
Preservative≤0.1% sodium azide
CommentsRecognizes multiple forms of calpastatin that are suggested to be a result of translation initiation from different start sites. Epitope is in domain IV (543-673) of human calpastatin. Reacts with calpastatin from several mammalian sources. Does not cross-react with calpain or calmodulin. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.
Lane, R.D., et al. 1992. Exper. Cell Res. 203, 5.