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205604 Anti-E-Cadherin (ECCD-2) Rat mAb (ECCD-2)

205604
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
R
Description
Overview

This product has been discontinued.



We are offering Anti-E-cadherin Antibody, clone DECMA-1 (Cat. No. MABT26) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






Recognizes the E-cadherin protein. Not suitable for inhibition assays of E-cadherin-dependent cell-cell contact.
Catalogue Number205604
Brand Family Calbiochem®
SynonymsAnti-Uvomorulin
References
ReferencesTakeichi, M. 1991. Science 251, 1451.
Takeichi, M. 1988. Development 102, 639.
Nagafuchi, A., et al. 1987. Nature 329, 341.
Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
Product Information
FormLyophilized
FormulationLyophilized from 10 mM TBS, 1% BSA, pH 7.4.
PreservativeNone
Applications
Application ReferencesFlow Cytometry Tegoshi, T., et al. 2005. APMIS 113, 91.
Key Applications Not Paraffin Sections
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesFrozen Sections (10 µg/ml)
Flow Cytometry (see application references)
Immunoblotting (10 µg/ml)
Paraffin Sections (not recommended)
Cell Inhibition Assays (not recommended)
Application CommentsDoes not inhibit E-cadherin-dependent cell-cell contact. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogenmouse liver E-cadherin fragment
ImmunogenMouse
CloneECCD-2
HostRat
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-E-Cadherin (ECCD-2) Rat mAb (ECCD-2) Certificates of Analysis

TitleLot Number
205604

References

Reference overview
Takeichi, M. 1991. Science 251, 1451.
Takeichi, M. 1988. Development 102, 639.
Nagafuchi, A., et al. 1987. Nature 329, 341.
Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-July-2007 RFH
SynonymsAnti-Uvomorulin
ApplicationFrozen Sections (10 µg/ml)
Flow Cytometry (see application references)
Immunoblotting (10 µg/ml)
Paraffin Sections (not recommended)
Cell Inhibition Assays (not recommended)
DescriptionRat monoclonal antibody purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the E-cadherin protein.
HostRat
Immunogen speciesMouse
Immunogenmouse liver E-cadherin fragment
CloneECCD-2
IsotypeIgG2a
Specieshuman, mouse
FormLyophilized
FormulationLyophilized from 10 mM TBS, 1% BSA, pH 7.4.
PreservativeNone
CommentsDoes not inhibit E-cadherin-dependent cell-cell contact. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
Toxicity Standard Handling
ReferencesTakeichi, M. 1991. Science 251, 1451.
Takeichi, M. 1988. Development 102, 639.
Nagafuchi, A., et al. 1987. Nature 329, 341.
Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
Application referencesFlow Cytometry Tegoshi, T., et al. 2005. APMIS 113, 91.