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MABN2728-25UL Anti-FoxA2/HNF-3b Antibody, clone 4C7

This Anti-FoxA2/HNF-3b, clone 4C7, Cat. No. MABN2728, is a mouse monoclonal antibody that detects FoxA2/HGN-3b and is tested for use in EMSA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

MABN2728-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2728-25UL
DescriptionAnti-FoxA2/HNF-3b Antibody, clone 4C7
Alternate Names
  • Hepatocyte nuclear factor-3beta
  • HNF-3 beta
  • FOXA2
  • Forkhead box A2
Background InformationHepatocyte nuclear factor-3beta (UniProt: Q9PWP8; also known as HNF-3 beta, FOXA2) is encoded by the HNF-3beta gene (Gene ID: 395539) in Chicken. FoxA2 belongs to the forkhead family of transcription factors that uses a modified helix-turn-helix motif to bind DNA as a monomer. Its DNA binding domain is localized in amino acids 151-245. It is expressed very early during development and is involved in the development of several tissues, including liver, pancreas, lung, prostate, and the nervous system. In mouse embryo, FoxA2 expression is initiated during gastrulation in the node, notochord, and floor plate. Its induction in neural plate cells is an early response to notochord-derived signals and its ectopic expression induces floor plate differentiation. In the ventral mesencephalon and cerebellum, it is co-expressed with Engrailed homeoproteins - En1 and En2, which raises the possibility that the two transcription factors interact to regulate common target genes. FoxA2 expression is observed in different regions of the developing nervous system. Along with FoxA1, it is reported to be crucial for the specification and differentiation of dopamine neurons during embryonic development. FoxA2 also functions as a suppressor of tumor metastasis by inhibition of epithelial-to-mesenchymal transition in human lung cancers. It is shown to be a key target of TGF-β1 in controlling epithelial-to-mesenchymal transition (EMT) in lung cancer cells. (Ref.: Rausa, FM., et al. (2020). Mol. Cell. Biol. 20(21); 8264-8282; Domanskyi, A., et al (2014). Front. Cell. Neurosci. 8; 275; Tang, Y., et al. (2011). Cell Res. 21(2); 316-326; Foucher, I., et al. (2003). Development 130(9); 1867-1876; Ericson, J., et al. (1996). Cell. 87(4); 661-673).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis Anti-FoxA2/HNF-3b, clone 4C7, Cat. No. MABN2728, is a mouse monoclonal antibody that detects FoxA2/HGN-3b and is tested for use in EMSA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Electrophoretic Mobility Shift Assay
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesTested Applications

Electrophoretic Mobility Shift Assay: A representative lot detected FoxA2/HNF-3b in Electrophoretic Mobility Shift Assay (EMSA) (Foucher, I., et al. (2003). Development. 130(9): 1867-76).

Immunofluorescence Analysis: A representative lot detected FoxA2/HNF-3b in Immunofluorescence applications (Camp, D., et al. (2014). Development. 141(20): 3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1): 132-146).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FoxA2/HNF-3b in Immunoprecipitation applications (van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).

Immunohistochemistry Applications: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Ericson, J., et al. (1996). Cell. 87(4):661-73; Kwon, O., et al. (1998). J Clin Invest. 101(10):2054-64; Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28; Camp, D., et al. (2014). Development. 141(20):3879-88; Hoelzl, M.A., et al. (2017). Dev Biol. 429(1):132-146; Amarnath, S., et al. (2017). J Cell Sci. 130(1):119-131).

Immunocytochemistry Analysis: A representative lot detected FoxA2/HNF-3b in Immunohistochemistry applications (Nagase, T., et al. (2009). Dev Dyn. 238(5):1118-30).

Western Blotting Analysis: A representative lot detected FoxA2/HNF-3b in Western Blotting applications (Rousa, F.M., et al. (2000). Mol Cell Biol. 20(21):8264-82; van den Brink, G.R., et al. (2001). Gastroenterology. 121(2):317-28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant Chicken Rhodopsin (1-6).
EpitopeUnknown
Clone4C7
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 4C7 is a mouse monoclonal antibody that detects Hepatocyte nuclear factor-3β (HNF-3β/FoxA2).
IsotypeIgG1κ
Species Reactivity
  • Chicken
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse, Chicken.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HNF-3beta
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed; 47.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FoxA2/HNF-3b, in HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2728-25UL 04065270321626