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205605 Anti-N-Cadherin Rat mAb (NCD-2)

205605
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
R
Description
Overview

This product has been discontinued.





Recognizes the N-cadherin protein. May be used to inhibit E-cadherin-dependent cell-cell contact.
Catalogue Number205605
Brand Family Calbiochem®
References
ReferencesTakeichi, M. 1991. Science 251, 1451.
Fujimori, T., et al. 1990. Development 110, 97.
Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
Matsunaga, M., et al. 1988. Neuron 1, 289.
Takeichi, M. 1988. Development 102, 639.
Hatta, K., et al. 1987. Dev. Biol. 120, 215.
Hatta, K., and Takeichi, M. 1986. Nature 320, 447.
Product Information
FormLyophilized
FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
Applications
Key Applications Not Paraffin Sections
Cell Inhibition Assays
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesCell Inhibition Assays (100 µg/ml)
Immunoblotting (10 µg/ml)
Immunohistochemistry (10 µg/ml)
Frozen Sections (10 µg/ml)
Paraffin Sections (not recommended)
Application CommentsSpecific for chicken and frog N-cadherin. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. The antibody may be used to inhibit N-cadherin-dependent cell-cell contact. Not suitable for use with paraffin-embedded sections, when used in immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogennervous tissue of chick embryos
ImmunogenChick Embryo
CloneNCD-2
HostRat
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-N-Cadherin Rat mAb (NCD-2) Certificates of Analysis

TitleLot Number
205605

References

Reference overview
Takeichi, M. 1991. Science 251, 1451.
Fujimori, T., et al. 1990. Development 110, 97.
Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
Matsunaga, M., et al. 1988. Neuron 1, 289.
Takeichi, M. 1988. Development 102, 639.
Hatta, K., et al. 1987. Dev. Biol. 120, 215.
Hatta, K., and Takeichi, M. 1986. Nature 320, 447.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-July-2007 RFH
ApplicationCell Inhibition Assays (100 µg/ml)
Immunoblotting (10 µg/ml)
Immunohistochemistry (10 µg/ml)
Frozen Sections (10 µg/ml)
Paraffin Sections (not recommended)
DescriptionMonoclonal antibody purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the N-cadherin protein.
HostRat
Immunogen speciesChick Embryo
Immunogennervous tissue of chick embryos
CloneNCD-2
IsotypeIgG2a
SpeciesXenopus, chicken
FormLyophilized
FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
CommentsSpecific for chicken and frog N-cadherin. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. The antibody may be used to inhibit N-cadherin-dependent cell-cell contact. Not suitable for use with paraffin-embedded sections, when used in immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
Toxicity Standard Handling
ReferencesTakeichi, M. 1991. Science 251, 1451.
Fujimori, T., et al. 1990. Development 110, 97.
Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
Matsunaga, M., et al. 1988. Neuron 1, 289.
Takeichi, M. 1988. Development 102, 639.
Hatta, K., et al. 1987. Dev. Biol. 120, 215.
Hatta, K., and Takeichi, M. 1986. Nature 320, 447.