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AP1054 Anti-Sphingosine Kinase 2 Rabbit pAb

Anti-Sphingosine Kinase 2 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SPHK2

AP1054
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~75 kD sphingosine kinase 2 protein in transfected HEK293 cells and breast and hepatic carcinoma tissue.
Catalogue NumberAP1054
Brand Family Calbiochem®
SynonymsAnti-SPHK2
Application Data
Detection of human sphingosine kinase 2 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with SphK2 expressing a c-Myc tag. Primary antibodies: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054) (lane 1) and antibody specific for the c-Myc tag (lane 2). Detection: chemiluminescence.

Detection of human sphingosine kinase 2 by staining paraffin sections. Samples: human breast carcinoma tissue. Primary antibody: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054). Detection: AEC.
References
ReferencesShu, X., et al. 2002.Mol. Cell. Biol. 22, 7758.
Xia, P., et al. 2002.J. Biol. Chem. 277, 7996.
Liu, H., et al. 2000.J. Biol. Chem. 275, 19513.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlBreast carcinoma
Preservative≤ 0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (1:1000)
Immunoblotting (1:100-500)
Paraffin Sections (1:50-100)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human SPHK2, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1054 0

Documentation

Anti-Sphingosine Kinase 2 Rabbit pAb Certificates of Analysis

TitleLot Number
AP1054

References

Reference overview
Shu, X., et al. 2002.Mol. Cell. Biol. 22, 7758.
Xia, P., et al. 2002.J. Biol. Chem. 277, 7996.
Liu, H., et al. 2000.J. Biol. Chem. 275, 19513.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-December-2008 JSW
SynonymsAnti-SPHK2
ApplicationELISA (1:1000)
Immunoblotting (1:100-500)
Paraffin Sections (1:50-100)
Application Data
Detection of human sphingosine kinase 2 by immunoblotting. Samples: whole cell lysate from HEK293 cells transfected with SphK2 expressing a c-Myc tag. Primary antibodies: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054) (lane 1) and antibody specific for the c-Myc tag (lane 2). Detection: chemiluminescence.

Detection of human sphingosine kinase 2 by staining paraffin sections. Samples: human breast carcinoma tissue. Primary antibody: Anti-Sphingosine Kinase 2 Rabbit pAb (Cat. No. AP1054). Detection: AEC.
DescriptionProtein G purified rabbit polyclonal antibody. Recognizes the ~75 kD sphingosine kinase 2 protein.
BackgroundSphingosine kinase (SphK) catalyzes the phosphorylation of the lipid, sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility, and differentiation. SphK is an important signaling enzyme that is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human SPHK2, conjugated to KLH
IsotypeIgG
Specieshuman
Positive controlBreast carcinoma
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤ 0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesShu, X., et al. 2002.Mol. Cell. Biol. 22, 7758.
Xia, P., et al. 2002.J. Biol. Chem. 277, 7996.
Liu, H., et al. 2000.J. Biol. Chem. 275, 19513.