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PC527 Anti-Survivin (Ab-1) (1-12) Rabbit pAb

Anti-Survivin (Ab-1) (1-12) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC527
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~16 kDa survivin protein in MOLT-4 cells.
  • Antibody Target Gene Symbol: BIRC5
  • Target Synonym: AAC-11, AP14, API4, EPR-1, SURVIVIN, SURVIVIN 2B, Survivin delta ex3, survivin40, TIAP
  • Entrez Gene Name: baculoviral IAP repeat-containing 5
  • Hu Entrez ID: 332 (Related Antibodies: 574660)
  • Mu Entrez ID: 11799
  • Rat Entrez ID: 64041
    		•
    Catalogue NumberPC527
    Brand Family Calbiochem®
    Application Data
    Detection of human survivin by immunoblotting. Sample: Whole cell lysate from MOLT4 cells. Primary antibody: Anti-Survivin (Ab-1) (1-12) Rabbit pAb (Cat. No. PC527) (1 µg/ml) pre-incubated without blocking petide (lane A) or with blocking peptide (lane B). Detection: chemiluminescence.
    References
    ReferencesKobayashi, K., 1999. Proc. Natl. Acad. Sci. USA. 88, 1457.
    Li, F., et al. 1998. Nature 396, 580.
    Ambrosini, G., et al. 1997. Nat. Med. 3, 917.
    Product Information
    FormLiquid
    FormulationIn PBS.
    Positive controlMOLT4 cells
    Preservative≤0.1% sodium azide
    Applications
    Key Applications Immunoblotting (Western Blotting)
    Application NotesImmunoblotting (1:500-1:1000)
    Application CommentsAntibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena synthetic peptide (MGAPTLPPAWQP) corresponding to amino acids 1-12 of human survivin
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Standard Handling
    Storage -20°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    PC527 0

    Documentation

    Anti-Survivin (Ab-1) (1-12) Rabbit pAb SDS

    Title

    Safety Data Sheet (SDS) 

    Anti-Survivin (Ab-1) (1-12) Rabbit pAb Certificates of Analysis

    TitleLot Number
    PC527

    References

    Reference overview
    Kobayashi, K., 1999. Proc. Natl. Acad. Sci. USA. 88, 1457.
    Li, F., et al. 1998. Nature 396, 580.
    Ambrosini, G., et al. 1997. Nat. Med. 3, 917.

    Brochure

    Title
    Caspases and other Apoptosis Related Tools Brochure
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision11-October-2007 RFH
    ApplicationImmunoblotting (1:500-1:1000)
    Application Data
    Detection of human survivin by immunoblotting. Sample: Whole cell lysate from MOLT4 cells. Primary antibody: Anti-Survivin (Ab-1) (1-12) Rabbit pAb (Cat. No. PC527) (1 µg/ml) pre-incubated without blocking petide (lane A) or with blocking peptide (lane B). Detection: chemiluminescence.
    DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~16 kDa survivin protein.
    BackgroundApoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members of the TNF family, and prevented by inhibitor of apoptosis (IAP) proteins. IAP proteins, the products of a conserved gene family, bind to and inhibit cell death proteases. One novel IAP protein was recently identified and has been designated survivin. Survivin, a ~16 kDa protein, interacts with the processed form of caspase-3 and inhibits its proteolytic activity. Survivin is predominantly expressed in the tissues of embryos, transformed cell lines, and in many human cancers and lymphomas.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (MGAPTLPPAWQP) corresponding to amino acids 1-12 of human survivin
    IsotypeIgG
    Specieshuman
    Positive controlMOLT4 cells
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAntibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesKobayashi, K., 1999. Proc. Natl. Acad. Sci. USA. 88, 1457.
    Li, F., et al. 1998. Nature 396, 580.
    Ambrosini, G., et al. 1997. Nat. Med. 3, 917.