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OP53 Anti-c-Fos (Ab-3) Mouse mAb (CS93.1.18)

Anti-c-Fos (Ab-3) Mouse mAb (CS93.1.18) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP53
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~50-62 kDa c-Fos protein in serum-stimulated NIH3T3 cells and A431 cells. Also recognizes v-fos.
Catalogue NumberOP53
Brand Family Calbiochem®
References
ReferencesRouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M., and Graham, W.R.,1987. Adv. Cancer Res. 49, 29.
Verma, I.M., and Sassone-Corsi, P., 1987. Cell 52, 513).
Muller, R., 1986. Biochim. Biophys. Acta 823, 207.
Verma, I.M., 1986. Trends Genet. 2, 93.
Greenberg, M., and Ziff, E., 1984. Nature, London 311, 433.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Negative controlGrowth-arrested cells
Positive controlSerum-stimulated NIH 3T3 cells or A431 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml; pepsin pre-treatment required)
Application CommentsThis antibody will react with both cellular and viral forms of Fos. The immunogen c-Fos (Peptide-2), Cat. No. PP10, is also available for competition studies. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
ImmunogenHuman
CloneCS93.1.18
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP53 0

Documentation

Anti-c-Fos (Ab-3) Mouse mAb (CS93.1.18) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-Fos (Ab-3) Mouse mAb (CS93.1.18) Certificates of Analysis

TitleLot Number
OP53

References

Reference overview
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M., and Graham, W.R.,1987. Adv. Cancer Res. 49, 29.
Verma, I.M., and Sassone-Corsi, P., 1987. Cell 52, 513).
Muller, R., 1986. Biochim. Biophys. Acta 823, 207.
Verma, I.M., 1986. Trends Genet. 2, 93.
Greenberg, M., and Ziff, E., 1984. Nature, London 311, 433.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
ApplicationImmunoblotting (1-5 µg/ml)
Paraffin Sections (1-10 µg/ml; pepsin pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-AG14 plasmacytoma cells. Recognizes the ~50-62 kDa Fos protein.
BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. c-Fos is induced by a large number of stimuli including from mitogens, differentiation-specific agents and pharmacological agents. Induction of the 62 kDa Fos protein is rapid but transient. The Fos protein is associated with a 39 kDa protein which has been identified as the protein product of the Jun proto-oncogene (c-jun). The Fos/Jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
CloneCS93.1.18
IsotypeIgG₁
Specieshuman, mouse
Positive controlSerum-stimulated NIH 3T3 cells or A431 cells
Negative controlGrowth-arrested cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsThis antibody will react with both cellular and viral forms of Fos. The immunogen c-Fos (Peptide-2), Cat. No. PP10, is also available for competition studies. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesRouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M., and Graham, W.R.,1987. Adv. Cancer Res. 49, 29.
Verma, I.M., and Sassone-Corsi, P., 1987. Cell 52, 513).
Muller, R., 1986. Biochim. Biophys. Acta 823, 207.
Verma, I.M., 1986. Trends Genet. 2, 93.
Greenberg, M., and Ziff, E., 1984. Nature, London 311, 433.