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NA70 Anti-p14ARF (Ab-2) Mouse mAb (14PO2)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NA70
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~14 kDa p14ARF protein in HeLa cells. Sold under license of U.S. Patent 6,482,929.
Catalogue NumberNA70
Brand Family Calbiochem®
References
ReferencesBates, S., et al. 1998. Nature 395, 124.
Product Information
DeclarationSold under license of U.S. Patent 6,482,929.
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Negative controlNormal sheep serum
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmnunoblotting (2-4 µg/ml)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human p14ARF (p16β)
ImmunogenHuman
Clone14PO2
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA70 0

Documentation

Anti-p14ARF (Ab-2) Mouse mAb (14PO2) SDS

Title

Safety Data Sheet (SDS) 

Anti-p14ARF (Ab-2) Mouse mAb (14PO2) Certificates of Analysis

TitleLot Number
NA70

References

Reference overview
Bates, S., et al. 1998. Nature 395, 124.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
ApplicationImmnunoblotting (2-4 µg/ml)
Immunofluorescence (0.5 µg/ml)
Immunoprecipitation (2 µg/mg lysate)
DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splonocytes with mouse myeloma cells. Recognizes the ~14 kDa p14ARF protein.
BackgroundThe INK4α-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. It binds to mdm2 and promotes the rapid degradation of mdm2 protein. This interaction is mediated by the exon 1-β-encoded N-terminal domain of p14ARF and a C-terminal region of mdm2. ARF-mediated mdm2 degradation is associated with mdm2 modification and concurrent p53 stabilization and accumulation. Deletion of the ARF-INK4α locus simultaneously impairs both the INK4α-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human p14ARF (p16β)
Clone14PO2
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlHeLa cells
Negative controlNormal sheep serum
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBates, S., et al. 1998. Nature 395, 124.