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14-530M-D BRAF

Recombinant human BRAF, amino-acids 417-766 (end) with N-terminal GST-tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

BRAF MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-530M-D
250 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-530M-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptionBRAF
OverviewRecombinant human BRAF, amino-acids 417-766 (end) with N-terminal GST-tag
References
Product Information
Presentation40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM Glutathione, 20% glycerol, and 3 mM DTT
Quality LevelMQ300
Applications
ApplicationRecombinant human BRAF, amino-acids 417-766 (end) with N-terminal GST-tag, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Concentration0.11 mg/ml
Purity≥50%
SourceSf9 insect cells
Specific Activity44 pmol/min/µg
Entrez Gene Number
Entrez Gene SummaryProtein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Gene Symbol
  • BRAF
  • BRAF1
  • B-Raf
  • MGC126806
  • RAFB1
  • p94
  • MGC138284
  • B-raf-1
Target Sub-FamilyTKL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P15056 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SIZE: 766 amino acids; 84437 Da
SUBUNIT: Interacts with RIT1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain and testis.
DISEASE: SwissProt: P15056 # Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. & Defects in BRAF are involved in a wide range of cancers. & Defects in BRAF are involved in lung cancer [MIM:211980]. & Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. & Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
SIMILARITY: SwissProt: P15056 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight66 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: Reaction was conducted in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 2 µg inactive MEK1 (BPS #40075), 5 µM ATP, and 0.1 – 1.6 ng/µl BRAF for 60 min at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size250 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-530M-D 04055977276602