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197216 Bcl-xL Assay Substrate, Fluorogenic

197216
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





A fluorescein-labeled Bad BH3 peptide that serves as an useful probe for investigating the nature of Bcl-xL- peptide interaction and as a substrate for high-throughput screening of Bcl-xL inhibitors based on fluorescence polarization. Interacts strongly with Bcl-xL with a Kd of 21.48 nM. The assay tolerates up to 8% DMSO.
Catalogue Number197216
Brand Family Calbiochem®
SynonymsAc-NLWAAQRYGRELRRMSDK(fluorescein)FVD-OH
References
ReferencesZhang, H., et al. 2002. Anal. Biochem. 307, 70
Product Information
FormYello lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₃₇H₁₉₂N₃₈O₃₈S₂
Chemical formulaC₁₃₇H₁₉₂N₃₈O₃₈S₂
Hygroscopic Hygroscopic
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Emission max.
Excitation max.
Peptide SequenceAc-Asn-Leu-Trp-Ala-Ala-Gln-Arg-Tyr-Gly-Arg-Glu-Leu-Arg-Arg-Met-Ser-Asp-Lys(FITC-I)-Phe-Val-Asp-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Bcl-xL Assay Substrate, Fluorogenic Certificates of Analysis

TitleLot Number
197216

References

Reference overview
Zhang, H., et al. 2002. Anal. Biochem. 307, 70

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-April-2008 RFH
SynonymsAc-NLWAAQRYGRELRRMSDK(fluorescein)FVD-OH
DescriptionA Fluorescein-labeled Bad BH3 peptide that serves as a useful probe for investigating the nature of Bcl-xL peptide interaction. Also useful in fluorescence polarization assays for high-throughput screening of Bcl-xL inhibitors. Reported to exhibit strong interaction with Bcl-xL (Kd = 21.48 nM). Fluorescence polarization assay shown to tolerate up to 8% DMSO. Excitation max: ~485 nm; emission max: ~530 nm.
FormYello lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₃₇H₁₉₂N₃₈O₃₈S₂
Peptide SequenceAc-Asn-Leu-Trp-Ala-Ala-Gln-Arg-Tyr-Gly-Arg-Glu-Leu-Arg-Arg-Met-Ser-Asp-Lys(FITC-I)-Phe-Val-Asp-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Toxicity Standard Handling
ReferencesZhang, H., et al. 2002. Anal. Biochem. 307, 70