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208697 Calmodulin, Biotinylated, Bovine Brain

208697
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



A ubiquitous Ca2+-binding protein that serves as a physiological effector of a wide range of biological processes. Useful for the study of calmodulin-binding proteins immobilized on nitrocellulose, by Western blot or dot blot. Calmodulin purified from bovine brain and biotinylated.

Note: this product cannot be used for animal treatment, in vivo research, or any contact procedure with livestock.
Catalogue Number208697
Brand Family Calbiochem®
SynonymsBiotin-CaM
References
ReferencesHernandez, E.O., et al. 1994. Tissue Cell 26, 849.
Kincaid, R.L., et al. 1988. Methods Enzymol. 159, 605.
Hubbard, M.J., and Klee, C.B. 1987. J. Biol. Chem. 262, 15062.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA, 0.02% NaN₃.
Applications
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, store in the refrigerator (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Calmodulin, Biotinylated, Bovine Brain SDS

Title

Safety Data Sheet (SDS) 

Calmodulin, Biotinylated, Bovine Brain Certificates of Analysis

TitleLot Number
208697

References

Reference overview
Hernandez, E.O., et al. 1994. Tissue Cell 26, 849.
Kincaid, R.L., et al. 1988. Methods Enzymol. 159, 605.
Hubbard, M.J., and Klee, C.B. 1987. J. Biol. Chem. 262, 15062.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-March-2015 JSW
SynonymsBiotin-CaM
DescriptionUseful for the detection of calmodulin-binding proteins immobilized on nitrocellulose in Western blot or dot blot protocols. Biotin-CaM is detectable with streptavidin-alkaline phosphatase conjugate (Cat. No. 189732). Calmodulin purified from bovine brain and biotinylated.

Note: This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.
FormLiquid
FormulationIn PBS, 1 mg/ml BSA, 0.02% NaN₃.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Protocol: Calmodulin, Biotinylated, Bovine Brain 10 ng of calcineurin were detected using 100 ng/ml of biotinylated calmodulin with the following method: 1. Immobilize the CaM-binding protein on nitrocellulose. 2. Prepare the reaction buffer: 50 mM Tris, pH 7.5, 200 mM NaCl, 500 µM CaCl2, 50 mM MgCl2, and 1% BSA. Block the nitrocellulose membrane by incubating at 20°C for 1 h with the reaction buffer. 3. Remove the blocking solution. Add the biotinylated calmodulin at 100 ng/ml in reaction buffer. Incubate at 20°C for 2 h. 4. Remove the biotinylated calmodulin solution. Wash 2 X 10 min in TBS/CaMg containing 0.05% TWEEN® 20, PROTEIN GRADE® Detergent, 10% Solution (Cat. No. 655206). 5. Incubate at 20°C for 30 min with Streptavidin, Alkaline Phosphatase Conjugated (Cat. No. 189732) diluted in the reaction buffer. 6. Wash 3 x 15 min with TBS/CaMg. Prepared substrate buffer: 100 mM Tris, 100 mM NaCl, pH 9.5, 50 mM MgCl2, and 0.5 mM CaCl2. Wash 1 x 15 min with substrate buffer. 7. Incubate for 20-30 min, protected from light, with NBT (330 mg/ml) and BCIP (0.165 mg/ml) in substrate buffer or use BCIP/NBT (Cat. No. 203790). 8. Wash extensively with H2O and air dry, protected from light.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, store in the refrigerator (4°C).
Toxicity Standard Handling
ReferencesHernandez, E.O., et al. 1994. Tissue Cell 26, 849.
Kincaid, R.L., et al. 1988. Methods Enzymol. 159, 605.
Hubbard, M.J., and Klee, C.B. 1987. J. Biol. Chem. 262, 15062.