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218820 Caspase-2 Substrate IV, Colorimetric

218820
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Description
Overview

This product has been discontinued.





A colorimetric substrate for the detection of caspase-2 (ICH-1). Cleavage is monitored colorimetrically at ~405 nm.
Catalogue Number218820
Brand Family Calbiochem®
SynonymsAc-VDVAD-pNA
References
ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Product Information
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₂₉H₄₂N₇O₁₂
Chemical formulaC₂₉H₄₂N₇O₁₂
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide SequenceAc-Val-Asp-Val-Ala-Asp-pNA
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-2 Substrate IV, Colorimetric Certificates of Analysis

TitleLot Number
218820

References

Reference overview
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-June-2008 RFH
SynonymsAc-VDVAD-pNA
DescriptionA colorimetric substrate for the detection of caspase-2 (ICH-1) activity (Km = 53 µM). May also serve as a substrate for caspase-3 (Km = 67 µM) and caspase-7 (Km = 200 µM). Cleavage is monitored by an increase in absorbance at 405 nm.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Recommended reaction conditions100 mM NaOAc, pH 6.2, 20% (v/v) glycerol, 5 mM DTT, 0.5 mM EDTA, 0.1% (w/v) BSA, 3-200 ng purified caspase-2, 10-100 mM Ac-VDVAD-pNA.
Chemical formulaC₂₉H₄₂N₇O₁₂
Peptide SequenceAc-Val-Asp-Val-Ala-Asp-pNA
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Protect from moisture
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Toxicity Standard Handling
ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.