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218754 Caspase-5 Substrate II, Fluorogenic

218754
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Description
Overview

This product has been discontinued.





A fluorogenic substrate for caspase-5 (ICErel-III, TY), caspase-1, and caspase-4. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
Catalogue Number218754
Brand Family Calbiochem®
SynonymsAc-WEHD-AFC
References
ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
Product Information
ATP CompetitiveN
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₃₈H₃₇F₃N₈O₁₁
Chemical formulaC₃₈H₃₇F₃N₈O₁₁
ReversibleN
Applications
Biological Information
Primary TargetA fluorogenic substrate for caspase-5 (ICErel-III, TY), caspase-1, and caspase-4
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceAc-Trp-Glu-His-Asp-AFC
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-5 Substrate II, Fluorogenic Certificates of Analysis

TitleLot Number
218754

References

Reference overview
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsAc-WEHD-AFC
DescriptionA fluorogenic substrate for caspase-5 (ICErel-III, TY), caspase-1, and caspase-4. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Chemical formulaC₃₈H₃₇F₃N₈O₁₁
Peptide SequenceAc-Trp-Glu-His-Asp-AFC
Purity≥95% by HPLC
SolubilityDMSO (50 mM)
Storage -20°C
Protect from moisture
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.