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219396 Cathepsin D, Bovine Spleen

219396
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Description
Overview

This product has been discontinued.





Native cathepsin D from bovine spleen. Cathepsin D is a major lysosomal aspartyl proteinase widely distributed in many cell types. It exists in two forms, a single polypeptide chain of 46 kDa, and a two-polypeptide chain form consisting of an H-chain of 34 kDa and an L-chain of 12 kDa.v
Catalogue Number219396
Brand Family Calbiochem®
References
ReferencesBerchem, G., et al. 2002. Oncogene 21, 5951.
Fortgens, P.H., et al. 1997. Immunopharmacology 36, 305.
Takahashi, T., and Tang, J. 1981. Methods Enzymol. 80 (Pt. C), 565.
Product Information
CAS number9025-26-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will digest acid-denatured hemoglobin in 30 minutes, at 37°C, pH 3.3, releasing peptides soluble in TCA, as measured by an increase in absorbance of 0.5 at 280 nm.
EC number3.4.23.5
FormLyophilized
FormulationLyophilized from 2 mM sodium phosphate buffer, pH 6.5.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥400 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Cathepsin D, Bovine Spleen SDS

Title

Safety Data Sheet (SDS) 

Cathepsin D, Bovine Spleen Certificates of Analysis

TitleLot Number
219396

References

Reference overview
Berchem, G., et al. 2002. Oncogene 21, 5951.
Fortgens, P.H., et al. 1997. Immunopharmacology 36, 305.
Takahashi, T., and Tang, J. 1981. Methods Enzymol. 80 (Pt. C), 565.

Brochure

Title
Cathepsins and Related Products Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-December-2010 JSW
DescriptionNative cathepsin D from bovine spleen. Cathepsin D is a major lysosomal aspartyl proteinase distributed in a wide variety of cells. Overexpression and alteration in secretion of cathepsin D are linked to tumor invasion and inflammation. The enzyme exists in two forms, a single chain form of 46 kDa, and a two polypeptide chain form with an H chain of 34 kDa and an L chain of 12 kDa.
FormLyophilized
FormulationLyophilized from 2 mM sodium phosphate buffer, pH 6.5.
CAS number9025-26-7
EC number3.4.23.5
Purity≥95% by SDS-PAGE
Specific activity≥400 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will digest acid-denatured hemoglobin in 30 minutes, at 37°C, pH 3.3, releasing peptides soluble in TCA, as measured by an increase in absorbance of 0.5 at 280 nm.
SolubilityH₂O or 2 mM Sodium Phosphate Buffer, pH 6.5. Please see vial label for lot-specific reconstitution volume.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBerchem, G., et al. 2002. Oncogene 21, 5951.
Fortgens, P.H., et al. 1997. Immunopharmacology 36, 305.
Takahashi, T., and Tang, J. 1981. Methods Enzymol. 80 (Pt. C), 565.