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219463 Cathepsin E, His•Tag®, Mouse, Recombinant, NSO Cells

219463
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, mouse cathepsin E fused at the C-terminus to a His•Tag® sequence and expressed NSO cells. Cathepsin E is an intracellular aspartic protease of the pepsin family, which has been reported to be involved in the cleavage of the Swedish mutant of amyloid precursor protein. Due to glycosylation, the apparent molecular mass of the dimer is ~100 kDa under non-reducing conditions. Note: Must be activated before use.
Catalogue Number219463
Brand Family Calbiochem®
References
ReferencesGruninger-Leitch, F., et al. 2000. Nat. Biotechnol. 18, 66.
Tatnell, P.J., et al. 1997. FEBS Lett. 408, 62.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-PLGL(3-DPA-diaminopropionyl)-AR-NH₂ at 25°C, pH 3.5.
EC number3.4.23.34
FormLiquid
FormulationIn 75 mM NaCl, 12.5 mM MES, 50% glycerol, pH 6.5, sterile-filtered.
Applications
Biological Information
Purity≥95 by SDS-PAGE
Specific Activity≥8 units/μg
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxins: ≤1.0 EU/µg enzyme
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Cathepsin E, His•Tag®, Mouse, Recombinant, NSO Cells Certificates of Analysis

TitleLot Number
219463

References

Reference overview
Gruninger-Leitch, F., et al. 2000. Nat. Biotechnol. 18, 66.
Tatnell, P.J., et al. 1997. FEBS Lett. 408, 62.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-June-2008 RFH
DescriptionRecombinant, mouse cathepsin E fused at the C-terminus to a His•Tag® sequence and expressed NSO cells. Cathepsin E is an intracellular aspartic protease of the pepsin family that has been reported to be involved in the cleavage of the Swedish mutant of amyloid precursor protein (APP). Due to glycosylation the apparent molecular weight of the dimer is ~100 kDa under non-reducing conditions. Requires activation prior to use.
FormLiquid
FormulationIn 75 mM NaCl, 12.5 mM MES, 50% glycerol, pH 6.5, sterile-filtered.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditionsActivation Protocol Pre-incubate Cathepsin E for 15 min. at room temperature, at a concentration of 1 µg/ml in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl. Activity Optimal activity is achieved in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl, room temperature.
EC number3.4.23.34
Purity≥95 by SDS-PAGE
ContaminantsEndotoxins: ≤1.0 EU/µg enzyme
Specific activity≥8 units/μg
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-PLGL(3-DPA-diaminopropionyl)-AR-NH₂ at 25°C, pH 3.5.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesGruninger-Leitch, F., et al. 2000. Nat. Biotechnol. 18, 66.
Tatnell, P.J., et al. 1997. FEBS Lett. 408, 62.