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219347 Cathepsin K Detection Kit

219347
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Detection Methods
Fluorescence
Description
Overview

This product has been discontinued.





Suitable for detection of cathepsin K in suspension or adherent cells. Detects cathepsin K using a cell-permeable cresyl violet-labeled cathepsin K substrate, which generates red fluorescence upon cleavage.
Catalogue Number219347
Brand Family Calbiochem®
Materials Required but Not Delivered Cultured cells with medium
Reagents to induce apoptosis or other experimental condition
15 ml polypropylene centrifuge tube (1 per sample)
Amber vials or polypropylene tubes for storage of 260X concentrate aliquots at -20°C
Slides and coverslips
Hemocytometer
Clinical centrifuge at 200 X g
37°C CO2 incubator
Pipette(s) capable of dispensing at 10, 50, 200, 300 µl, and 1 ml
ddH2O, up to 2 ml needed
Phosphate buffered saline (PBS) pH 7.4, up to 100 ml needed
Dimethyl sulfoxide (DMSO)
Trypsin-EDTA
Ice buckets or a refrigerator to maintain cells at 2°C to 8°C
Fluorescence microscope with appropriate filters: excitation 550 nm, emission >610 nm for Cathepsin K fluorogenic substrate; excitation at 480 nm and emission at >540 nm for AO; and if Hoechst is used, a UV-filter with excitation at 365 nm, emission at 480 nm.
References
ReferencesBoonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473.
Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
Kas J. et al. 2000. Int. J. Biol. Markers 15, 84.
Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98.
Frosch B.A., et al. 1999. APMIS 107, 28.
Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71.
Bossard M.J., 1996. J. Biol. Chem. 271, 12524.
Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135.
Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185.
Buhling F., et al. Adv. Exp. Med. Biol. 477, 281.
Leung-Toung R., et al. Med. Chem. 9, 979.
Product Information
Detection methodFluorescence
FormatFluorescence microscopy
Kit containsLabeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol.
Applications
Biological Information
Assay time4-6 h
Sample TypeCells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Intended useThe Calbiochem® Cathepsin K Detection Kit is intended for the non-quantitative measurement of Cathepsin K activity in intact cells.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsLabeled Cathepsin K Substrate, Hoechst 33342 Stain, Acridine Orange, and a user protocol.
Specifications

Documentation

Cathepsin K Detection Kit Certificates of Analysis

TitleLot Number
219347

References

Reference overview
Boonacker E., and Van Noorden, C.J.F. 2001. J. Histochem. Cytochem. 49, 1473.
Bank U., et al. 2000. Adv. Exp. Med. Biol. 477, 349.
Buhling F., et al. 2000. Adv. Exp. Med. Biol. 477, 241.
Gerber A., et al. 2000. Adv. Exp. Med. Biol. 477, 287.
Kas J. et al. 2000. Int. J. Biol. Markers 15, 84.
Turk B., et al. 2000. Biochim. Biophys. Acta 1477, 98.
Frosch B.A., et al. 1999. APMIS 107, 28.
Van Noorden C.J.F., et al. 1997. Anal. Biochem. 252, 71.
Bossard M.J., 1996. J. Biol. Chem. 271, 12524.
Guinec N., et al. 1993. Biol. Chem. Hoppe-Seyler 374, 1135.
Traganos F., and Darzynkiewicz, Z. 1994. Methods Cell Biol. 41, 185.
Buhling F., et al. Adv. Exp. Med. Biol. 477, 281.
Leung-Toung R., et al. Med. Chem. 9, 979.

Brochure

Title
Kit SourceBook - 2nd Edition EURO

User Protocols

Title
User Protocol