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219344 Cathepsin S, Human Spleen

219344
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Description
OverviewNative cathepsin S from human spleen. A major lysosomal cysteine proteinase that mediates degradation of MHC class II-invariant chain complexes in human and mouse antigen presenting cells. Cathepsin S secreted by activated macrophages has the ability to process myelin basic protein and amyloid β peptide which may be relevant to the pathogenesis of Alzheimer′s disease. It can hydrolyze elastin at both acidic and neutral pH.

This product has been discontinued.





Note: 1 mU = 1 milliunit.
Catalogue Number219344
Brand Family Calbiochem®
References
ReferencesLiuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
Kirschke, H., et al. 1989. Biochem. J. 264, 467.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze Z-VVR-AMC in 10 min at 25°C, in 100 mM KPO₄, 5 mM EDTA, pH 6.5 (the assay is standardized such that 0.041 nmol AMC gives a reading of 1000 RFU.
EC number3.4.22.27
FormLyophilized
FormulationLyophilized from 100 mM sodium acetate, 1 mM EDTA, pH 5.5.
Applications
Biological Information
Purity≥90% by SDS-PAGE
SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HBC, HCV, and HIV.
Specific Activity≥50 mU/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Cathepsin S, Human Spleen SDS

Title

Safety Data Sheet (SDS) 

Cathepsin S, Human Spleen Certificates of Analysis

TitleLot Number
219344

References

Reference overview
Liuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
Kirschke, H., et al. 1989. Biochem. J. 264, 467.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-September-2007 JSW
DescriptionNative cathepsin S from human spleen. A major lysosomal cysteine protease preferentially expressed in macrophages and microglia. Mediates degradation of MHC Class II-invariant chain complexes in human and mouse antigen presenting cells. Cathepsin S secreted by activated macrophages may be relevant to the pathogenesis of Alzheimer's disease as a result of its ability to process myelin basic protein and β amyloid (Aβ) peptide. Cathepsin S can also hydrolyze elastin at both acidic and neutral pH.
FormLyophilized
FormulationLyophilized from 100 mM sodium acetate, 1 mM EDTA, pH 5.5.
SourcePrepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HBC, HCV, and HIV.
EC number3.4.22.27
Purity≥90% by SDS-PAGE
Specific activity≥50 mU/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze Z-VVR-AMC in 10 min at 25°C, in 100 mM KPO₄, 5 mM EDTA, pH 6.5 (the assay is standardized such that 0.041 nmol AMC gives a reading of 1000 RFU.
SolubilityAqueous buffers. Please refer to the label for concentration prior to lyophilization.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Toxicity Standard Handling
ReferencesLiuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
Kirschke, H., et al. 1989. Biochem. J. 264, 467.