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234133 Collagenase, Paralithodes camtschatica Hepatopancreas

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
234133
  
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Overview

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Description
Overview

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Mixture of collagenolytic proteases that can be separated and characterized by FPLC. Has high activity against different types of collagen, especially calf skin collagen Type III and bovine lens capsule collagen Type IV, that are resistant to clostridial collagenases. Excellent, general-purpose protease that has chymotrypsin-, trypsin-, and elastase-like specificity and cleaves both standard synthetic and protein substrates. Ability to cleave native, insoluble collagen makes it particularly suitable for isolation of viable cells from tissues. Recommended for harvesting of endothelial cells.
Note: 1 KU = 1000 units.
Catalogue Number234133
Brand Family Calbiochem®
References
ReferencesKlimova, O.A., et al. 1993. Biochem. Biophys. Res. Commun. 195, 758.
Klimova, O.A., et al. 1990. Biochem. Biophys. Res. Commun. 100, 1411.
Product Information
CAS number9001-12-1
Activity≥1,000 Mandl units/mg dry weight
Unit of DefinitionOne Mandl unit is defined as the amount of enzyme that will release 1.0 µmol Leu equivalent from collagen in 5 h at 37°C, pH 7.5.
EC number3.4.24.3
FormLyophilized
Hygroscopic Hygroscopic
PI2.3, temperature optimum 37°C
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42/43

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-24-37

Do not breathe dust.
Avoid contact with skin.
Wear suitable gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
234133 0

Documentation

Collagenase, Paralithodes camtschatica Hepatopancreas SDS

Title

Safety Data Sheet (SDS) 

Collagenase, Paralithodes camtschatica Hepatopancreas Certificates of Analysis

TitleLot Number
234133

References

Reference overview
Klimova, O.A., et al. 1993. Biochem. Biophys. Res. Commun. 195, 758.
Klimova, O.A., et al. 1990. Biochem. Biophys. Res. Commun. 100, 1411.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-April-2008 RFH
DescriptionMixture of collagenolytic proteases that can be separated and characterized by FPLC. Excellent general purpose protease with chymotrypsin-, trypsin-, and elastase-like specificity. Cleaves both standard synthetic and protein substrates. Has high activity against different types of collagen, especially calf skin collagen Type III and bovine lens capsule collagen Type IV, that are resistant to clostridial collagenases. Has the ability to cleave native, insoluble collagen, making it particularly suitable for the isolation of intact viable cells from tissues. Recommended for harvesting of endothelial cells. Stable at pH 6.5-8.5, and has an optimal pH and temperature of 7.7 and 37°C, respectively.
FormLyophilized
CAS number9001-12-1
EC number3.4.24.3
Activity≥1,000 Mandl units/mg dry weight
Unit definitionOne Mandl unit is defined as the amount of enzyme that will release 1.0 µmol Leu equivalent from collagen in 5 h at 37°C, pH 7.5.
SolubilityAqueous Buffers (1 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 2481
ReferencesKlimova, O.A., et al. 1993. Biochem. Biophys. Res. Commun. 195, 758.
Klimova, O.A., et al. 1990. Biochem. Biophys. Res. Commun. 100, 1411.