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71865 Gateway® Nova pET-62-DEST™ Expression System

71865
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Generating expression plasmids starting from the Gateway® Nova pET-62-DEST™ vector requires recombination with pENTR clones that carry their own ribosome binding site (RBS) and translation initiation site. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will be fused to consecutive C-terminal His•Tag® and Strep•Tag® II coding sequences. The consecutive purification tags enable the use of consecutive "gentle" purification procedures. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.
Catalogue Number71865
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71865 0