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Merck

D7058

DirectLoad Wide Range DNA Marker

ready-to-use marker for DNA electrophoresis

Synonym(s):

agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105335
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−20°C
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Quality Segment

form

liquid

usage

100 uses

solubility

water: soluble

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Other Notes

Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Sigma′s DirectLoad Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC


Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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