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Merck

A4550

Agar

Type A, suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
9002-18-0
UNSPSC Code:
50405901
eCl@ss:
42040102
EC Number:
232-658-1
NACRES:
NA.21
MDL number:
MFCD00081288

Product Name

Agar, Type A, suitable for plant cell culture

SMILES string

O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

biological source

algae (Rhodophyceae)

type

Type A

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 32-38 °C (1.5%)

gel strength

>650 g/cm2

application(s)

agriculture

storage temp.

room temp

Quality Level

200

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Application

Type A agar has been used:
  • to perform metal(loid)-related phenotyping of the Arabidopsis thaliana metal-sensitive mutant strains
  • as a component of Murashige and Skoog (MS) media to culturing Arabidopsis thaliana seeds
  • to culture Cicer arietinum L. (Chickpea) seeds for Agrobacterium rhizogenes mediated root transformation

General description

Agar (Type A) is extracted from agarocytes of the red alga Rhodophyceae. It is a polysaccharide complex, consisting of a neutral gelling fraction, agarose, and a non-gelling fraction, agaropectin. Type-A agar forms a thermoreversible gel, and is most suitable for plant cell culture.

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000512491
0000512493
0000512492
0000512492
0000512493

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Zhihang Feng et al.
Plant physiology, 184(1), 428-442 (2020-07-01)
Mg2+ is among the most abundant divalent cations in living cells. In plants, investigations on magnesium (Mg) homeostasis are restricted to the functional characterization of Mg2+ transporters. Here, we demonstrate that the splicing factors SUPPRESSORS OF MEC-8 AND UNC-52 1
Shimpei Uraguchi et al.
Frontiers in plant science, 11, 503-503 (2020-06-06)
For researchers in the plant metal field, the agar reagent used for the solid plate medium is a problematic factor because application of different agar types and even a different lot of the same agar type can mask the plant
Extraction and characterization of agar from Australian Pterocladia lucida
Chiovitti A, et al.,
Journal of Applied Phycology, 16(1), 41-48 (2004)
Antoine Desrut et al.
Journal of experimental botany, 71(22), 7301-7315 (2020-08-30)
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using
Pooja Rani Aggarwal et al.
Plant methods, 14, 55-55 (2018-07-11)
Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics studies in this crop. Here, we present an optimized and
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