- GABAergic signaling facilitates breast cancer metastasis by promoting ERK1/2-dependent phosphorylation.
GABAergic signaling facilitates breast cancer metastasis by promoting ERK1/2-dependent phosphorylation.
Cancer letters (2014-03-25)
Depu Zhang, Xiaowei Li, Ziming Yao, Chuanfei Wei, Nannan Ning, Jingxin Li
PMID24657659
ABSTRACT
The present study aims to determine the role of γ-aminobutyric acid (GABA) signaling molecules in breast cancer metastasis. Our results reveal that GABAergic system exists in breast cancer cells. Both the GABA synthetic enzyme. (GAD65/67) and GABAB receptor are expressed in 4T1 mouse breast cancer cells, MCF-7 human breast cancer cells and human breast cancer tissue. Baclofen, a GABABR agonist, significantly promoted 4T1 cells invasion and migration in vitro and metastasis in vivo, an event that was attenuated by GABABR antagonist CGP55845. Baclofen-induced breast cancer metastasis was mediated by ERK1/2 pathway.
MATERIALS
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Sigma-Aldrich
L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Supelco
L-Glutamine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland