I4640

Human IgG4 Kappa, Recombinant Protein

Name: Human IgG4 Kappa, Recombinant Protein
Brand: SIGMA

purified immunoglobulin, buffered aqueous solution

Synonym(s):

Human IgG4 Protein

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Human IgG4 Kappa, Recombinant Protein, purified immunoglobulin, buffered aqueous solution

biological source

human

conjugate

unconjugated

antibody form

purified immunoglobulin

assay

>95% (SDS-PAGE)

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

Quality Level

300

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.

Application

The purified IgG4, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunoblotting, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.

General description

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta and is vital for immune defense of the neonate against infections. IgG4, κ recombinant protein is obtained by expression of a phage library in a human cell line and purified by affinity chromatography.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Rafael D Melani et al.

Journal of proteome research, 21(1), 274-288 (2021-12-09)

Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy

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