R6148
RNase A solution
Synonym(s):
GenElute™ RNAse A
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About This Item
NACRES:
NA.52
UNSPSC Code:
12352204
biological source
bovine pancreas
form
liquid
specific activity
1500 units/mL
3000 units/mL
concentration
20 mg/mL in Tris buffer
technique(s)
DNA purification: suitable
storage temp.
room temp
Quality Level
Gene Information
bovine ... RNASE1(282340), RNASE1(280930)
Application
RNase A solution has been used:
- in apoptosis assay of Hep-2 cells
- for the colorimetric quantification of methylated DNA
- for DNA and RNA extraction
- in DNA laddering analysis
- in cell cycle analysis
- as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry
Biochem/physiol Actions
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
General description
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Preparation Note
For usage requirements, please refer to the kit procedure.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Seasonal changes in broodstock spawning performance and egg quality in ballan wrasse (Labrus bergylta)
Grant B, et al.
Aquaculture (Amsterdam, Netherlands), 464(, 505-514 (2016)
Taotao Shou et al.
Molecular medicine reports, 19(2), 1049-1055 (2018-11-30)
MicroRNAs (miRNAs) are acknowledged as essential regulators in human cancer types, including glioblastoma (GBM). However, the functions of microRNA‑3666 (miR‑3666) in GBM remain unclear. In the present study, it was identified that the expression of miR‑3666 was significantly downregulated in
Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies
Scheraga HA, et al.
Methods in Enzymology, 341, 189-221 (2001)
Nimesulide inhibits proliferation and induces apoptosis of pancreatic cancer cells by enhancing expression of PTEN
Chu M, et al.
Experimental and Therapeutic Medicine, 16(1), 370-376 (2018)
Homogeneous electrochemiluminescence biosensor for the detection of RNase A activity and its inhibitor
Jiancong N, et al.
Analytical Chemistry, 91, 14751-14756 (2019)
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