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ST1546 Anti-CBS Mouse mAb (3E1)

Anti-CBS Mouse mAb (3E1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cystathione Β-Synthase

ST1546
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~75 kDa CBS protein in HeLa and MCF-7 cells and human hepatocellular carcinoma tissue.
      Catalogue NumberST1546
      Brand Family Calbiochem®
      SynonymsAnti-Cystathione Β-Synthase
      Application Data
      Detection of human CBS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CBS by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CBS by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human hepatocellular carcinoma tissue. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (3 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesHennig, B., and Diener, M., 2009. Br. J. Pharmacol. 158, 1263.
      Lee, M., et al. 2009. Neurobiol. Aging 30, 1523.
      Martin, G,R., et al. 2009. Dig. Liver Dis. 42, 103.
      Wallace, J,L., et al. 2009. Gastroenterology 137, 569.
      Xu, G,Y., et al. 2009. Mol. Pain 5, 44.
      Majtan, T., et al. 2008. J. Biol. Chem. 283, 34588.
      Wallace, J,L., et al. 2007. FASEB J. 21, 4070.
      Schicho, R., et al. 2006. Gastroenterology 131 1542.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, MCF-7 cells, Human hepatocellular carcinoma tissue
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human CBS, expressed as a GST fusion protein
      Clone3E1
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1546 0

      Documentation

      References

      Reference overview
      Hennig, B., and Diener, M., 2009. Br. J. Pharmacol. 158, 1263.
      Lee, M., et al. 2009. Neurobiol. Aging 30, 1523.
      Martin, G,R., et al. 2009. Dig. Liver Dis. 42, 103.
      Wallace, J,L., et al. 2009. Gastroenterology 137, 569.
      Xu, G,Y., et al. 2009. Mol. Pain 5, 44.
      Majtan, T., et al. 2008. J. Biol. Chem. 283, 34588.
      Wallace, J,L., et al. 2007. FASEB J. 21, 4070.
      Schicho, R., et al. 2006. Gastroenterology 131 1542.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-November-2010 RFH
      SynonymsAnti-Cystathione Β-Synthase
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human CBS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CBS by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CBS by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human hepatocellular carcinoma tissue. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa CBS protein.
      BackgroundCBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine β-synthase deficiency (CBSD), which can lead to homocystinuria.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human CBS, expressed as a GST fusion protein
      Clone3E1
      IsotypeIgG2a
      Specieshuman, rat
      Positive controlHeLa cells, MCF-7 cells, Human hepatocellular carcinoma tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesHennig, B., and Diener, M., 2009. Br. J. Pharmacol. 158, 1263.
      Lee, M., et al. 2009. Neurobiol. Aging 30, 1523.
      Martin, G,R., et al. 2009. Dig. Liver Dis. 42, 103.
      Wallace, J,L., et al. 2009. Gastroenterology 137, 569.
      Xu, G,Y., et al. 2009. Mol. Pain 5, 44.
      Majtan, T., et al. 2008. J. Biol. Chem. 283, 34588.
      Wallace, J,L., et al. 2007. FASEB J. 21, 4070.
      Schicho, R., et al. 2006. Gastroenterology 131 1542.