MABE945 Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8

MABE945
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, ChIP, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE945
      DescriptionAnti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8
      Alternate Names
      • E3 ubiquitin-protein ligase UHRF1
      • Inverted CCAAT box-binding protein of 90 kDa
      • Nuclear protein 95
      • Nuclear zinc finger protein Np95
      • HuNp95
      • hNp95
      • RING finger protein 106
      • Transcription factor ICBP90
      • Ubiquitin-like PHD and RING finger domain-containing protein 1
      • hUHRF1
      • Ubiquitin-like-containing PHD and RING finger domains protein 1
      Background InformationUbiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1.
      Key Applications
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected E3 ubiquitin-protein ligase UHRF1 in overexpressed lysate (Martin Walsh, Mount Sinai School of Medicine).
      Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in NTera2 cells (Martin Walsh, Mount Sinai School of Medicine).
      Biological Information
      ImmunogenRecombinant protein corresponding to human E3 ubiquitin-protein ligase UHRF1.
      Clone7C8
      Concentration1 mg/mL
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UHRF1
      • ICBP90
      • NP95
      • RNF106
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight90 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected E3 ubiquitin-protein ligase UHRF1 in HeLa cells.
      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies