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MCYT1-190K-SPX MILLIPLEX® Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1

MILLIPLEX® Qualified assays undergo rigourous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 68 analytes in mouse serum, plasma, and cell culture supernatants.

The following analytes in this kit are not compatible with the MAGPIX® System: sFasL, IL-16, sFas, IL-20, sCD137, sICAM-1, CXCL16, sRAGE, CCL25/TECK, BAFF, GDF-15, FLT3L, CHI3L1, MCP-2, MCP-3, EPO, Fracktalkine, IL-31, and Betacellulin.

MCYT1-190K-SPX
96-well plate  96 well plate
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      Overview

      Replacement Information
      Description
      Catalogue NumberMCYT1-190K-SPX
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1
      OverviewMILLIPLEX® kits offer the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

      Each MILLIPLEX® kit includes:
      • Quality controls (QCs) to qualify assay performance
      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
      • Detection antibody cocktails designed to yield consistent analyte profiles within panel

      In addition, each panel and kit meet stringent manufacturing criteria to ensure batch to-batch reproducibility. MILLIPLEX® Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1 is part of the most versatile system available for exosome research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically verify, and build the most comprehensive library available for protein detection and quantitation.
      Background InformationMouse models are fundamental in the field of biomedical research, serving as essential tools in preclinical and translational studies across various fields such as immunology, cancer, and infectious diseases. Cytokines, chemokines, and growth factors are key mediators of immune system functions capable of signaling through autocrine, paracrine, and endocrine mechanisms.  Their pleiotropic immunomodulatory properties allow these biomolecules to react to diverse stimuli and regulate the immune response, either promoting or inhibiting inflammation.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-assay: <10%
      • Inter-assay: <15% (Exodus <20%)
      Panel TypeMAGNETIC Circulating Cancer
      Quality LevelMQ200
      Applications
      ApplicationMILLIPLEX® Qualified assays undergo rigourous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 68 analytes in mouse serum, plasma, and cell culture supernatants.

      The following analytes in this kit are not compatible with the MAGPIX® System: sFasL, IL-16, sFas, IL-20, sCD137, sICAM-1, CXCL16, sRAGE, CCL25/TECK, BAFF, GDF-15, FLT3L, CHI3L1, MCP-2, MCP-3, EPO, Fracktalkine, IL-31, and Betacellulin.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight or two-hour incubation assay.
      • This assay requires 25 µL of 1:2 diluted plasma or serum 25 μL cell culture supernatant per well.
      • This kit may be used for the analysis of any or all of the Mouse Cytokine/Chemokine/Growth Factor Expanded Panel 1 analytes in mouse tissue/cell lysate and culture supernatant samples and serum or plasma samples.
      Biological Information
      Species Reactivity
      • Mouse
      Analytes Available
      • EGF
      • Eotaxin/CCL11
      • FGF-2
      • G-CSF
      • GM-CSF
      • IFN-α
      • IFNβ
      • IFNγ
      • IL-10
      • IL-12 (p40)
      • IL-12(p70)
      • IL-13
      • IL-15
      • IL-17
      • IL-17F
      • IL-18
      • IL-1α
      • IL-1β
      • IL-2
      • IL-22
      • IL-28B/IFNλ3
      • IL-3
      • IL-4
      • IL-5
      • IL-6
      • IL-7
      • IL-9
      • IP-10/CXCL10
      • KC/CXCL1
      • LIF
      • LIX
      • MCP-1/CCL2
      • M-CSF
      • MIG
      • MIP-1α
      • MIP-1β
      • RANTES
      • TNFα
      • VEGF-A
      • IL-33
      • MIP-2
      Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities for individual analytes.
      Standard Curve Range
      • Refer to kit protocol for standard curve ranges for individual analytes.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MCYT1-190K-SPX 04065272030359

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